C57BL/6JCya-Zfp536em1/Cya
Common Name:
Zfp536-KO
Product ID:
S-KO-19225
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Zfp536-KO
Strain ID
KOCMP-243937-Zfp536-B6J-VA
Gene Name
Product ID
S-KO-19225
Gene Alias
9630010P11Rik; Znf536; mKIAA0390
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
7
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Zfp536em1/Cya mice (Catalog S-KO-19225) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000056338
NCBI RefSeq
NM_172385
Target Region
Exon 4
Size of Effective Region
~3.2 kb
Detailed Document
Overview of Gene Research
Zfp536, also known as Zinc Finger Protein 536, is likely involved in multiple biological processes. It has been associated with cell lineage differentiation, potentially playing a role in regulating the development of specific cell types. It may participate in gene regulatory networks and could be involved in pathways related to tissue development and homeostasis [1,2,3,4]. Genetic models, such as mouse models, can be valuable for further exploring its functions.
In the development of perinatal mouse skin, Zfp536 was detected as a putative key driver gene for the papillary fibroblast trajectory, indicating its role in dermal fibroblast lineage differentiation [1]. In the developing cerebral cortex, a sense lncRNA, SenZfp536, which is transcribed downstream of and partially overlaps with Zfp536, cis-regulates Zfp536. Knocking down SenZfp536 increased the proliferation of cortical neural progenitor cells (NPCs), and overexpressing Zfp536 in cortical NPCs reversed this enhanced proliferation, suggesting Zfp536's role in suppressing NPC self-renewal [2]. In tooth initiation, Zfp536 was found near the Sox2:Tfap2a/Tfap2b interface in the mandibular epithelium, and disruption of domain-specific transcription factors led to changes in the expression of genes like Zfp536, affecting tooth initiation site patterning [3]. Computational analysis identified Zfp536 as potentially part of the core transcriptional regulatory networks of Sox2+ dental epithelial stem cells in both embryonic and postnatal stages [4].
In summary, Zfp536 is involved in cell lineage differentiation processes in skin, cerebral cortex, and tooth development. Mouse models have been crucial in uncovering its role in these biological processes, providing insights into the molecular mechanisms underlying tissue development and potentially related disease conditions such as abnormal skin or tooth development, as well as neural development-related disorders [1,2,3,4].
References:
1. Lee, Hanjae, Kim, So Young, Kwon, Nak-Jung, Kwon, Ohsang, Kim, Jong-Il. 2023. Single-Cell and Spatial Transcriptome Analysis of Dermal Fibroblast Development in Perinatal Mouse Skin: Dynamic Lineage Differentiation and Key Driver Genes. In The Journal of investigative dermatology, 144, 1238-1250.e11. doi:10.1016/j.jid.2023.11.008. https://pubmed.ncbi.nlm.nih.gov/38072389/
2. Tian, Kuan, Wang, Andi, Wang, Junbao, Liu, Ying, Zhou, Yan. 2020. Transcriptome Analysis Identifies SenZfp536, a Sense LncRNA that Suppresses Self-renewal of Cortical Neural Progenitors. In Neuroscience bulletin, 37, 183-200. doi:10.1007/s12264-020-00607-2. https://pubmed.ncbi.nlm.nih.gov/33196962/
3. Shao, Fan, Phan, An-Vi, Yu, Wenjie, Van Otterloo, Eric, Cao, Huojun. 2024. Transcriptional programs of Pitx2 and Tfap2a/Tfap2b controlling lineage specification of mandibular epithelium during tooth initiation. In PLoS genetics, 20, e1011364. doi:10.1371/journal.pgen.1011364. https://pubmed.ncbi.nlm.nih.gov/39052671/
4. Shao, Fan, Van Otterloo, Eric, Cao, Huojun. 2023. Computational identification of key transcription factors for embryonic and postnatal Sox2+ dental epithelial stem cell. In bioRxiv : the preprint server for biology, , . doi:10.1101/2023.12.22.573158. https://pubmed.ncbi.nlm.nih.gov/38187542/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen