C57BL/6JCya-Kcnb1em1/Cya
Common Name
Kcnb1-KO
Product ID
S-KO-19306
Backgroud
C57BL/6JCya
Strain ID
KOCMP-16500-Kcnb1-B6J-VB
Status
When using this mouse strain in a publication, please cite “Kcnb1-KO Mouse (Catalog S-KO-19306) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Kcnb1-KO
Strain ID
KOCMP-16500-Kcnb1-B6J-VB
Gene Name
Product ID
S-KO-19306
Gene Alias
Shab, Kv2.1, Kcr1-1
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 2 (Mouse)
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Transcript ID
ENSMUST00000207917
NCBI Transcript ID
NM_008420
Target Region
Exon 2
Size of Effective Region
~2.3 kb
Overview of Gene Research
KCNB1, encoding the α subunit of the delayed rectifier voltage-dependent potassium channel Kv 2.1, is a key player in multiple biological processes. It conducts a major delayed rectifier current in the brain, pancreas, and cardiovascular system and is involved in apoptotic programs associated with oxidative stress [1].
In animal models, KCNB1 null mice showed impaired neuronal migration of glutamatergic neurons in the neocortex, with persistent migratory defects in adulthood, along with disrupted morphology and synaptic connectivity. These mice also developed a seizure phenotype, anxiety, and compulsive behavior [3]. In zebrafish, a Kcnb1 loss-of-function mutation affected inner ear development at multiple levels, including otic vesicle expansion, otolith formation, and the proliferation and differentiation of mechanosensory cells [2].
In conclusion, KCNB1 is essential for normal neuronal and inner ear development. The study of KCNB1 knockout mouse models has revealed its crucial role in the development of neurological phenotypes such as epilepsy and behavioral disorders, providing important insights into the underlying mechanisms of these diseases [2,3].
References：
1. Sesti, Federico, Wu, Xilong, Liu, Shuang. . Oxidation of KCNB1 K(+) channels in central nervous system and beyond. In World journal of biological chemistry, 5, 85-92. doi:10.4331/wjbc.v5.i2.85. https://pubmed.ncbi.nlm.nih.gov/24921000/
2. Jedrychowska, Justyna, Gasanov, Eugene V, Korzh, Vladimir. 2020. Kcnb1 plays a role in development of the inner ear. In Developmental biology, 471, 65-75. doi:10.1016/j.ydbio.2020.12.007. https://pubmed.ncbi.nlm.nih.gov/33316259/
3. Bortolami, Alessandro, Yu, Wei, Forzisi, Elena, Rasin, Mladen-Roko, Sesti, Federico. 2022. Integrin-KCNB1 potassium channel complexes regulate neocortical neuronal development and are implicated in epilepsy. In Cell death and differentiation, 30, 687-701. doi:10.1038/s41418-022-01072-2. https://pubmed.ncbi.nlm.nih.gov/36207442/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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