C57BL/6JCya-Zfp36l1em1/Cya
Common Name:
Zfp36l1-KO
Product ID:
S-KO-19370
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Zfp36l1-KO
Strain ID
KOCMP-12192-Zfp36l1-B6J-VA
Gene Name
Product ID
S-KO-19370
Gene Alias
Berg36; Brf1; D530020L18Rik; ERF1; TIS11b; cMG1
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
12
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Zfp36l1em1/Cya mice (Catalog S-KO-19370) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000021552
NCBI RefSeq
NM_007564
Target Region
Exon 2
Size of Effective Region
~1.6 kb
Detailed Document
Overview of Gene Research
Zfp36l1, a member of the RNA-binding zinc finger protein 36 (ZFP36) family, contains tandem CCCH-type zinc-finger motifs with RNA-binding property. It enhances the turnover of mRNAs containing AU-rich elements (AREs) in their 3'-untranslated regions (3'UTR), thus playing a crucial role in post-transcriptional regulation of numerous physiological processes, including cell transition, differentiation, and immune regulation [2,3].
In the context of diseases, liver-specific Zfp36l1-deficient (L1LKO) mice were protected from alcohol-induced hepatic steatosis, injury, and inflammation, likely by stabilizing Fgf21 mRNA [2]. In osteosarcoma, knockdown of Zfp36l1 promoted cell migration and lung metastasis by activating TGF-β signaling and increasing SDC4 expression, as Zfp36l1 regulated SDC4 mRNA decay through AREs in its 3'UTR [4]. In small cell lung cancers (SCLCs), loss-of-function screen showed Zfp36l1 is required for LSD1 inhibitor sensitivity. LSD1 represses Zfp36l1, and upon LSD1 inhibition, Zfp36l1 expression is restored, blocking SCLC neuroendocrine differentiation by destabilizing SOX2 and INSM1 mRNAs [1].
In conclusion, Zfp36l1 is a key regulator in post-transcriptional mRNA regulation. Studies using gene-knockout mouse models have revealed its significance in multiple disease areas, such as alcoholic liver disease, osteosarcoma metastasis, and SCLC neuroendocrine plasticity, providing insights into potential therapeutic targets for these diseases.
References:
1. Chen, Hsiao-Yun, Durmaz, Yavuz T, Li, Yixiang, Signoretti, Sabina, Oser, Matthew G. 2022. Regulation of neuroendocrine plasticity by the RNA-binding protein ZFP36L1. In Nature communications, 13, 4998. doi:10.1038/s41467-022-31998-7. https://pubmed.ncbi.nlm.nih.gov/36008402/
2. Bathula, Chandra S, Chen, Jian, Kumar, Rahul, Saini, Yogesh, Patial, Sonika. 2021. ZFP36L1 Regulates Fgf21 mRNA Turnover and Modulates Alcoholic Hepatic Steatosis and Inflammation in Mice. In The American journal of pathology, 192, 208-225. doi:10.1016/j.ajpath.2021.10.017. https://pubmed.ncbi.nlm.nih.gov/34774847/
3. Rynne, Jennifer, Ortiz-Zapater, Elena, Bagley, Dustin C, Adcock, Ian M, Martinez-Nunez, Rocio T. 2023. The RNA binding proteins ZFP36L1 and ZFP36L2 are dysregulated in airway epithelium in human and a murine model of asthma. In Frontiers in cell and developmental biology, 11, 1241008. doi:10.3389/fcell.2023.1241008. https://pubmed.ncbi.nlm.nih.gov/37928904/
4. Ma, Mengjun, Zhuang, Jiahao, Li, Hongyu, Wu, Yanfeng, Shen, Huiyong. 2023. Low expression of ZFP36L1 in osteosarcoma promotes lung metastasis by inhibiting the SDC4-TGF-β signaling feedback loop. In Oncogene, 43, 47-60. doi:10.1038/s41388-023-02880-7. https://pubmed.ncbi.nlm.nih.gov/37935976/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen