C57BL/6JCya-Ubap1em1/Cya
Common Name:
Ubap1-KO
Product ID:
S-KO-19373
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Ubap1-KO
Strain ID
KOCMP-67123-Ubap1-B6J-VC
Gene Name
Product ID
S-KO-19373
Gene Alias
2700092A01Rik; NAG20; UBAP-1; Ubap
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
4
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ubap1em1/Cya mice (Catalog S-KO-19373) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000072866
NCBI RefSeq
NM_023305
Target Region
Exon 3
Size of Effective Region
~1.6 kb
Detailed Document
Overview of Gene Research
Ubap1, or ubiquitin-associated protein 1, is one of the subunits of the endosomal sorting complex required for transport I (ESCRT-I). It binds to ubiquitin-tagged proteins, playing a crucial role in endosome sorting and is involved in endosomal trafficking pathways. Genetic models, such as knock-in and knockout mice, are valuable for studying Ubap1's function [1,2,3,4].
In Ubap1+/E176Efx23 knock-in mice (a model of SPG80), the animals showed normal appearance at birth but developed progressive hind limb dysfunction later. Molecularly, there was loss of neurons in the spinal cord, accumulation of ubiquitinated proteins, and disrupted endosome-mediated vesicular trafficking, as indicated by changes in Rab5 and Rab7 distributions [1]. Conditional disruption of Ubap1 in mice caused severe brain dysplasia and prenatal ventriculomegaly by disrupting adherens junctions and polarity of radial glial cells, reducing neural progenitor cell proliferation and inducing precocious differentiation [2]. In zebrafish, in vivo down-regulation of Ubap1 led to abnormal morphology, inhibited motor neuron outgrowth, decreased mobility, and shorter lifespan. In cell cultures, patient-derived truncated forms of UBAP1 caused aberrant endosome clustering, enlargement, and cytoplasmic accumulation of ubiquitinated proteins, and disruption of Ubap1 in cultured cortical neurons from transgenic Ubap1flox mice led to dysregulation of early endosome processing and ubiquitinated protein sorting [3].
In conclusion, Ubap1 is essential for endosome sorting and endosomal trafficking. Mouse models, including KO/CKO models, have revealed its significance in neurodegenerative disorders like hereditary spastic paraplegia (such as SPG80) and in brain development, providing insights into the molecular pathogenesis and potential for therapeutic agent screening [1,2,3].
References:
1. Shimozono, Keisuke, Nan, Haitian, Hata, Takanori, Koizumi, Schuichi, Takiyama, Yoshihisa. 2022. Ubap1 knock-in mice reproduced the phenotype of SPG80. In Journal of human genetics, 67, 679-686. doi:10.1038/s10038-022-01073-6. https://pubmed.ncbi.nlm.nih.gov/35962060/
2. Lu, Danping, Zhi, Yiqiang, Su, Huizhen, Wang, Ning, Xu, Dan. 2024. ESCRT-I protein UBAP1 controls ventricular expansion and cortical neurogenesis via modulating adherens junctions of radial glial cells. In Cell reports, 43, 113818. doi:10.1016/j.celrep.2024.113818. https://pubmed.ncbi.nlm.nih.gov/38402586/
3. Lin, Xiang, Su, Hui-Zhen, Dong, En-Lin, Wang, Ning, Chen, Wan-Jin. . Stop-gain mutations in UBAP1 cause pure autosomal-dominant spastic paraplegia. In Brain : a journal of neurology, 142, 2238-2252. doi:10.1093/brain/awz158. https://pubmed.ncbi.nlm.nih.gov/31203368/
4. Stefani, Flavia, Zhang, Ling, Taylor, Sandra, Pickering-Brown, Stuart, Woodman, Philip. 2011. UBAP1 is a component of an endosome-specific ESCRT-I complex that is essential for MVB sorting. In Current biology : CB, 21, 1245-50. doi:10.1016/j.cub.2011.06.028. https://pubmed.ncbi.nlm.nih.gov/21757351/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen