C57BL/6JCya-Hsh2dem1/Cya
Common Name:
Hsh2d-KO
Product ID:
S-KO-19392
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Hsh2d-KO
Strain ID
KOCMP-209488-Hsh2d-B6J-VB
Gene Name
Product ID
S-KO-19392
Gene Alias
ALX; Hsh2
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
8
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Hsh2dem1/Cya mice (Catalog S-KO-19392) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000072097
NCBI RefSeq
NM_197944
Target Region
Exon 4
Size of Effective Region
~0.8 kb
Detailed Document
Overview of Gene Research
Hsh2d, also known as hematopoietic SH2 domain containing, is involved in multiple biological processes. It may participate in inflammatory/immune-related biological processes, as several studies indicate its association with pathways relevant to these functions [3].
In acute lymphoblastic leukaemia, specifically T-cell acute lymphoblastic leukaemia (T-ALL), Hsh2d expression is downregulated compared to B-cell ALL. Overexpression of Hsh2d in HuT-78 cells inhibits CD28-mediated IL-2 activation, and its expression is necessary for HuT-78 cells to be resistant to methotrexate (MTX), suggesting its importance in T-ALL's resistance to MTX [1]. In colon cancer cells, Hsh2d is highly upregulated in doxorubicin-surviving proliferative cells and is correlated with U-ISGF3-related genes, potentially contributing to chemoresistance [2].
In murine experimental colitis, macrophages with p38α deficiency show upregulated Hsh2d in colon tissues, indicating its role in the immune process during colitis [4]. In cervical squamous cell carcinoma, Hsh2d is part of an mRNA-lncRNA signature related to immune infiltration, which can predict patient survival [5].
In summary, Hsh2d is associated with immune-related functions and plays important roles in various disease conditions such as leukaemia, colon cancer, colitis, and cervical squamous cell carcinoma. Its involvement in drug resistance and immune-related signatures provides potential research targets for understanding disease mechanisms and developing new treatment strategies.
References：
1. Wang, Jing, Xiong, Yiying. 2020. HSH2D contributes to methotrexate resistance in human T‑cell acute lymphoblastic leukaemia. In Oncology reports, 44, 2121-2129. doi:10.3892/or.2020.7772. https://pubmed.ncbi.nlm.nih.gov/33000278/
2. Trzaskoma, Pawel, Jung, SeolKyoung, Kanno, Yuka, O'Shea, John J, Chow, Carson C. 2024. Doxorubicin resistance involves modulation of interferon signaling, transcriptional bursting, and gene co-expression patterns of U-ISGF3-related genes. In Neoplasia (New York, N.Y.), 58, 101071. doi:10.1016/j.neo.2024.101071. https://pubmed.ncbi.nlm.nih.gov/39405604/
3. Armstrong, David A, Chen, Youdinghuan, Dessaint, John A, Christensen, Brock C, Ashare, Alix. 2019. DNA Methylation Changes in Regional Lung Macrophages Are Associated with Metabolic Differences. In ImmunoHorizons, 3, 274-281. doi:10.4049/immunohorizons.1900042. https://pubmed.ncbi.nlm.nih.gov/31356157/
4. Chen, Wei, Liang, Rui, Yi, Youcai, Zhu, Jinshui, Zhang, Jing. 2022. P38α deficiency in macrophages ameliorates murine experimental colitis by regulating inflammation and immune process. In Pathology, research and practice, 233, 153881. doi:10.1016/j.prp.2022.153881. https://pubmed.ncbi.nlm.nih.gov/35430508/
5. Mao, Yifang, Chen, Run, Xia, Meng, Huang, Jiaming, He, Mian. 2021. Identification of an immune-based mRNA-lncRNA signature for overall survival in cervical squamous cell carcinoma. In Future oncology (London, England), 17, 2365-2380. doi:10.2217/fon-2020-1153. https://pubmed.ncbi.nlm.nih.gov/33724869/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen