C57BL/6JCya-Gstt1em1/Cya
Common Name:
Gstt1-KO
Product ID:
S-KO-19397
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Gstt1-KO
Strain ID
KOCMP-14871-Gstt1-B6J-VC
Gene Name
Product ID
S-KO-19397
Gene Alias
Gstt1-1
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
10
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Gstt1em1/Cya mice (Catalog S-KO-19397) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000001713
NCBI RefSeq
NM_008185
Target Region
Exon 3
Size of Effective Region
~1.6 kb
Detailed Document
Overview of Gene Research
Gstt1, glutathione S-transferase theta 1, encodes an enzyme responsible for the detoxification of potentially harmful substances such as air pollution, drugs, pesticides, and tobacco [2]. It plays a role in protecting cells from oxidative stress by being part of the glutathione S-transferases enzyme group [1].
A meta-analysis indicated that GSTT1 polymorphisms may not be correlated with pre-eclampsia risk [1]. However, GSTT1 homozygous deletion was associated with a higher susceptibility to pancreatic cancer [3]. Also, in Caucasians, the GSTT1 null genotype was associated with prostate cancer risk [4]. Simultaneous deletion of Gstm1 and Gstt1 in CBA/CaJ mice led to more profound hearing loss under cisplatin treatment, suggesting Gstt1 protects auditory hair cells from cisplatin-induced ototoxicity [5]. GSTT1-null genotype was associated with increased bladder cancer risk in Caucasians [6]. The frequency of Gstt1 in gallbladder cancer patients was high before treatment but decreased after treatment [7]. In solid organ and hematopoietic stem cell transplantation, GSTT1 donor-recipient mismatch is associated with immune-related issues [8]. The GSTT1 null genotype was associated with increased coronary heart disease risk, and there was a positive interaction between the GSTT1 null genotype and smoking status [9]. In Asians, the GSTT1 null genotype was associated with increased lung cancer risk, especially in the Chinese population [10].
In conclusion, Gstt1 is crucial for detoxification and protecting cells from oxidative stress. Studies using gene-deletion models, such as the simultaneous deletion of Gstm1 and Gstt1 in mice, have revealed its role in various disease conditions including cancer, pre-eclampsia, cisplatin-induced ototoxicity, and coronary heart disease. These findings contribute to understanding disease mechanisms and may guide future research in disease treatment and prevention.
References:
1. Li, Lin, Wang, Ao, Ke, Yi. . GSTM1 and GSTT1 polymorphisms and risk of preeclampsia: a system review and meta-analysis. In The journal of maternal-fetal & neonatal medicine : the official journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians, 36, 2237623. doi:10.1080/14767058.2023.2237623. https://pubmed.ncbi.nlm.nih.gov/37469043/
2. Nakanishi, Giovana, Bertagnolli, Laísa S, Pita-Oliveira, Murilo, Cirino, Heithor S, Rodrigues-Soares, Fernanda. 2022. GSTM1 and GSTT1 polymorphisms in healthy volunteers - a worldwide systematic review. In Drug metabolism reviews, 54, 37-45. doi:10.1080/03602532.2022.2036996. https://pubmed.ncbi.nlm.nih.gov/35103568/
3. Cui, Jun-Peng, Lin, Mei-Yi, Liu, Zheng-Hao, Liu, Bao-Lin. . Association between GSTT1 Homozygous Deletion and Risk of Pancreatic Cancer: A Meta Analysis. In Chinese medical sciences journal = Chung-kuo i hsueh k'o hsueh tsa chih, 34, 205-210. doi:10.24920/003490. https://pubmed.ncbi.nlm.nih.gov/31648673/
4. Zhou, Tian-Biao, Drummen, Gregor P C, Jiang, Zong-Pei, Qin, Yuan-Han. 2014. GSTT1 polymorphism and the risk of developing prostate cancer. In American journal of epidemiology, 180, 1-10. doi:10.1093/aje/kwu112. https://pubmed.ncbi.nlm.nih.gov/24907267/
5. Li, Peipei, Liu, Ziyi, Wang, Jinpeng, Gao, Jiangang, Fu, Xiaolong. . Gstm1/Gstt1 is essential for reducing cisplatin ototoxicity in CBA/CaJ mice. In FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 36, e22373. doi:10.1096/fj.202200324R. https://pubmed.ncbi.nlm.nih.gov/35621716/
6. Yu, Cui, Hequn, Chen, Longfei, Liu, Chao, Li, Xiongbing, Zu. . GSTM1 and GSTT1 polymorphisms are associated with increased bladder cancer risk: Evidence from updated meta-analysis. In Oncotarget, 8, 3246-3258. doi:10.18632/oncotarget.13702. https://pubmed.ncbi.nlm.nih.gov/27911277/
7. Fang, Jianming, Fang, Fazhuang, Huang, Jianxin, Zhang, Xiaodong, Wang, Hongjuan. 2022. GSTM1 and GSTT1 Gene Polymorphisms with Gallbladder Carcinoma. In Cellular and molecular biology (Noisy-le-Grand, France), 68, 64-68. doi:10.14715/cmb/2022.68.8.11. https://pubmed.ncbi.nlm.nih.gov/36800835/
8. Aguilera, Isabel, Sousa, Jose Manuel, Núñez-Roldán, Antonio. 2013. Clinical relevance of GSTT1 mismatch in solid organ and hematopoietic stem cell transplantation. In Human immunology, 74, 1470-3. doi:10.1016/j.humimm.2013.06.004. https://pubmed.ncbi.nlm.nih.gov/23792056/
9. Song, Yadong, Shan, Zhilei, Luo, Cheng, Jiang, Xiaoming, Liu, Liegang. 2016. Glutathione S-Transferase T1 (GSTT1) Null Polymorphism, Smoking, and Their Interaction in Coronary Heart Disease: A Comprehensive Meta-Analysis. In Heart, lung & circulation, 26, 362-370. doi:10.1016/j.hlc.2016.07.005. https://pubmed.ncbi.nlm.nih.gov/27686690/
10. Zhang, Wen-Ping, Yang, Chen, Xu, Ling-Jun, Song, Liang, He, Xiao-Feng. . Individual and combined effects of GSTM1, GSTT1, and GSTP1 polymorphisms on lung cancer risk: A meta-analysis and re-analysis of systematic meta-analyses. In Medicine, 100, e26104. doi:10.1097/MD.0000000000026104. https://pubmed.ncbi.nlm.nih.gov/34190143/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen