C57BL/6JCya-Mtrf1lem1/Cya
Common Name:
Mtrf1l-KO
Product ID:
S-KO-19416
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Mtrf1l-KO
Strain ID
KOCMP-108853-Mtrf1l-B6J-VC
Gene Name
Product ID
S-KO-19416
Gene Alias
9130004K12Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
10
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Mtrf1lem1/Cya mice (Catalog S-KO-19416) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000019908
NCBI RefSeq
NM_175374
Target Region
Exon 3
Size of Effective Region
~1.2 kb
Detailed Document
Overview of Gene Research
Mtrf1l, a member of the mitochondrial release factor family, is a crucial mitochondrial translation factor. Mitochondrial translation, where mtDNA-encoded genes are translated into proteins, is vital for mitochondrial function and biogenesis, and Mtrf1l coordinates the translation termination phase [3].
In bovine oocytes, the transcript level of Mtrf1l was found to be significantly different (P˂0.01) between germinal vesicle stage (GV) oocytes with greater developmental competence from medium follicles and those with less developmental competence from small follicles, indicating its potential role in oocyte developmental competence [1]. Through large-scale coevolution analysis, a novel protein-protein interaction (PPI) between LYRM1-Mtrf1l was identified, which may help predict the molecular functions and biological processes Mtrf1l is involved in [2]. Also, HEMK1, a mammalian enzyme, can methylate the glutamine residue of the GGQ motif of Mtrf1l, although the functional significance of this modification in cell growth and mitochondrial protein homeostasis in HeLa cells under normal culture conditions seems dispensable [4].
In conclusion, Mtrf1l is an important mitochondrial translation factor. Its role in oocyte developmental competence in bovine and potential involvement in protein-protein interactions and post-translational modifications as revealed through these studies suggest its significance in mitochondrial-related biological processes. However, more research, potentially including gene knockout or conditional knockout models, is needed to fully understand its functions in specific disease areas.
References:
1. Nemcova, Lucie, Jansova, Denisa, Vodickova-Kepkova, Katerina, Machatkova, Marie, Kanka, Jiri. 2016. Detection of genes associated with developmental competence of bovine oocytes. In Animal reproduction science, 166, 58-71. doi:10.1016/j.anireprosci.2016.01.004. https://pubmed.ncbi.nlm.nih.gov/26811294/
2. Pei, Jimin, Zhang, Jing, Cong, Qian. . Human mitochondrial protein complexes revealed by large-scale coevolution analysis and deep learning-based structure modeling. In Bioinformatics (Oxford, England), 38, 4301-4311. doi:10.1093/bioinformatics/btac527. https://pubmed.ncbi.nlm.nih.gov/35881696/
3. Yokokawa, Takumi, Kido, Kohei, Suga, Tadashi, Hayashi, Tatsuya, Fujita, Satoshi. . Exercise-induced mitochondrial biogenesis coincides with the expression of mitochondrial translation factors in murine skeletal muscle. In Physiological reports, 6, e13893. doi:10.14814/phy2.13893. https://pubmed.ncbi.nlm.nih.gov/30369085/
4. Fang, Qi, Kimura, Yusuke, Shimazu, Tadahiro, Iwasaki, Shintaro, Shinkai, Yoichi. 2022. Mammalian HEMK1 methylates glutamine residue of the GGQ motif of mitochondrial release factors. In Scientific reports, 12, 4104. doi:10.1038/s41598-022-08061-y. https://pubmed.ncbi.nlm.nih.gov/35260756/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen