C57BL/6JCya-Dynlrb1em1/Cya
Common Name:
Dynlrb1-KO
Product ID:
S-KO-19418
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Dynlrb1-KO
Strain ID
KOCMP-67068-Dynlrb1-B6J-VC
Gene Name
Product ID
S-KO-19418
Gene Alias
2010012N15Rik; 2010320M17Rik; 9430076K19Rik; DNLC2A; Dncl2a; km23-1
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
2
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Dynlrb1em1/Cya mice (Catalog S-KO-19418) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000150602
NCBI RefSeq
NM_001291108
Target Region
Exon 3
Size of Effective Region
~2.4 kb
Detailed Document
Overview of Gene Research
Dynlrb1, also known as Dynein Light Chain Roadblock-Type 1, is an essential component of the dynein complex. The cytoplasmic dynein motor complex, of which Dynlrb1 is a part, is crucial for transporting essential signals and organelles from the cell periphery to the perinuclear region, thus being vital for the survival and function of highly polarized cells like neurons [1]. It is also involved in multiple signaling pathways and may play a role in axonal transport of lysosomes and retrograde signaling endosomes [1].
Homozygous Dynlrb1 null mice are not viable and die during early embryonic development [1]. Heterozygous or adult knockdown animals display reduced neuronal growth, and selective depletion of Dynlrb1 in proprioceptive neurons compromises their survival [1]. Conditional depletion of Dynlrb1 in sensory neurons causes deficits in several signaling pathways, including β -catenin subcellular localization, and severely impairs the axonal transport of lysosomes and retrograde signaling endosomes [1]. In addition, knockdown of Dynlrb1 in human CRC cells reduces cell migration, suggesting its role in cancer cell invasion [2].
In conclusion, Dynlrb1 is essential for dynein-mediated transport and neuronal survival. Studies using Dynlrb1 knockout mouse models have revealed its critical role in early embryonic development, neuronal growth and survival, as well as in certain signaling pathways. The findings also hint at its potential involvement in neurodegenerative diseases and cancer cell invasion, providing valuable insights into the underlying biological mechanisms and potential disease-related functions.
References:
1. Terenzio, Marco, Di Pizio, Agostina, Rishal, Ida, Fisher, Elizabeth M C, Fainzilber, Mike. 2020. DYNLRB1 is essential for dynein mediated transport and neuronal survival. In Neurobiology of disease, 140, 104816. doi:10.1016/j.nbd.2020.104816. https://pubmed.ncbi.nlm.nih.gov/32088381/
2. Raza, Asif, Pandey, Madhu S, Jin, Qunyan, Mulder, Kathleen M. 2019. km23-1/DYNLRB1 regulation of MEK/ERK signaling and R-Ras in invasive human colorectal cancer cells. In Cell biology international, 44, 155-165. doi:10.1002/cbin.11215. https://pubmed.ncbi.nlm.nih.gov/31393067/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen