C57BL/6JCya-Fabp12em1/Cya
Common Name:
Fabp12-KO
Product ID:
S-KO-19422
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Fabp12-KO
Strain ID
KOCMP-75497-Fabp12-B6J-VC
Gene Name
Product ID
S-KO-19422
Gene Alias
1700008G05Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
3
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Fabp12em1/Cya mice (Catalog S-KO-19422) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000029043
NCBI RefSeq
NM_029310
Target Region
Exon 4
Size of Effective Region
~1.7 kb
Detailed Document
Overview of Gene Research
Fabp12, a member of the fatty acid-binding protein (FABP) family, is involved in reversibly binding intracellular hydrophobic ligands and trafficking them across cellular compartments [2]. It has the same structure as other FABP genes and is located in a cluster with FABP4/5/8/9 within a 300,000 bp chromosomal region [3]. FABP12 orthologs are found in mammals. It plays a role in lipid-related bioenergetics and is associated with the PPARγ pathway [1,4].
In prostate cancer, FABP12 is preferentially amplified and/or overexpressed in metastatic tumors compared to primary tumors. It concurrently triggers metastatic phenotypes like epithelial-to-mesenchymal transition (EMT), leading to increased cell motility and invasion, and lipid bioenergetics, such as increased fatty acid uptake and accumulation, as well as ATP production from fatty acid β-oxidation [1]. Also, the FABP12-Slug-Survivin pathway drives docetaxel resistance in prostate cancer cells [4].
In rat models, FABP12 is expressed in the retina and testis, with a different expression pattern from FABP9 in testicular germ cells, suggesting distinct roles during spermatogenesis [3]. Additionally, in a study on anaphylactic shock due to adverse drug reactions, a negative causal relationship was found between FABP12 and anaphylactic shock [5].
In conclusion, Fabp12 is crucial in lipid-related bioenergetics and plays significant roles in processes such as prostate cancer metastasis and drug resistance, as well as in the development of the retina and testis in mammals. The research on Fabp12 using model-based studies, like those in rat models for expression analysis and in prostate cancer research, helps in understanding its functions and its implications in disease conditions [1,3,4,5].
References:
1. Liu, Rong-Zong, Choi, Won-Shik, Jain, Saket, Lemieux, Hélène, Godbout, Roseline. 2020. The FABP12/PPARγ pathway promotes metastatic transformation by inducing epithelial-to-mesenchymal transition and lipid-derived energy production in prostate cancer cells. In Molecular oncology, 14, 3100-3120. doi:10.1002/1878-0261.12818. https://pubmed.ncbi.nlm.nih.gov/33031638/
2. Smathers, Rebecca L, Petersen, Dennis R. . The human fatty acid-binding protein family: evolutionary divergences and functions. In Human genomics, 5, 170-91. doi:. https://pubmed.ncbi.nlm.nih.gov/21504868/
3. Liu, Rong-Zong, Li, Xiaodong, Godbout, Roseline. 2008. A novel fatty acid-binding protein (FABP) gene resulting from tandem gene duplication in mammals: transcription in rat retina and testis. In Genomics, 92, 436-45. doi:10.1016/j.ygeno.2008.08.003. https://pubmed.ncbi.nlm.nih.gov/18786628/
4. Liu, Rong-Zong, Garg, Mansi, Yang, Xiao-Hong, Godbout, Roseline. 2024. Docetaxel-Induced Cell Death Is Regulated by a Fatty Acid-Binding Protein 12-Slug-Survivin Pathway in Prostate Cancer Cells. In International journal of molecular sciences, 25, . doi:10.3390/ijms25179669. https://pubmed.ncbi.nlm.nih.gov/39273616/
5. Zhang, Yu, Zhang, Rusheng, Li, Cunyu, Peng, Guoping. . Exploring the causal association between fatty acid-binding proteins and anaphylactic shock due to adverse reactions to medications: A two-sample Mendelian randomization study. In Medicine, 104, e42171. doi:10.1097/MD.0000000000042171. https://pubmed.ncbi.nlm.nih.gov/40324257/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen