C57BL/6JCya-Atp6v0d1em1/Cya
Common Name:
Atp6v0d1-KO
Product ID:
S-KO-19427
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Atp6v0d1-KO
Strain ID
KOCMP-11972-Atp6v0d1-B6J-VB
Gene Name
Product ID
S-KO-19427
Gene Alias
Ac39; Atp6d; P39; VATX; Vma6
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
8
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Atp6v0d1em1/Cya mice (Catalog S-KO-19427) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000013304
NCBI RefSeq
NM_013477
Target Region
Exon 3
Size of Effective Region
~1.7 kb
Detailed Document
Overview of Gene Research
Atp6v0d1, encoding the ATPase H+ transporting V0 subunit D1, is a key subunit of vacuolar-type ATPase (V-ATPase) in lysosome [2]. V-ATPase is responsible for maintaining lysosomal acidification, which is crucial for many cellular processes such as protein degradation, nutrient recycling, and cell-death regulation [2,5]. It is also involved in pathways like the ATP6V0D1-STAT3 pathway related to alkaliptosis [1,3].
In pancreatic ductal adenocarcinoma (PDAC) cells, JTC801-mediated increase in ATP6V0D1 protein stability enhances its interaction with STAT3, sustaining lysosome homeostasis and promoting alkaliptosis. Pharmacological or genetic inhibition of STAT3 restores alkaliptosis sensitivity in ATP6V0D1-deficient cells in vitro and in mouse models. Clinically, high ATP6V0D1 expression correlates with prolonged PDAC patient survival [1]. In ovarian cancer, ATP6V0D1 can mediate the down-regulation of ABCB1, a multidrug-resistance protein, and inducing ATP6V0D1-dependent alkaliptosis may be a strategy against paclitaxel-resistant ovarian cancer cells [4].
In summary, Atp6v0d1 is vital for lysosomal function and homeostasis. Studies using in vitro and in vivo models, especially those with genetic manipulation of Atp6v0d1, have revealed its role in alkaliptosis-related cancer cell death mechanisms. These findings may offer potential therapeutic strategies for treating pancreatic and ovarian cancers [1,4].
References:
1. Chen, Fangquan, Zhu, Shan, Kang, Rui, Tang, Daolin, Liu, Jiao. 2022. ATP6V0D1 promotes alkaliptosis by blocking STAT3-mediated lysosomal pH homeostasis. In Cell reports, 42, 111911. doi:10.1016/j.celrep.2022.111911. https://pubmed.ncbi.nlm.nih.gov/36640329/
2. Zhou, Xiaoqing, Zhao, Shaoyang, Liu, Tingting, Tu, Pengfei, Zeng, Kewu. 2022. Schisandrol A protects AGEs-induced neuronal cells death by allosterically targeting ATP6V0d1 subunit of V-ATPase. In Acta pharmaceutica Sinica. B, 12, 3843-3860. doi:10.1016/j.apsb.2022.06.013. https://pubmed.ncbi.nlm.nih.gov/36213534/
3. Chen, Fangquan, Kang, Rui, Liu, Jiao, Tang, Daolin. 2023. Mechanisms of alkaliptosis. In Frontiers in cell and developmental biology, 11, 1213995. doi:10.3389/fcell.2023.1213995. https://pubmed.ncbi.nlm.nih.gov/37601110/
4. Chen, Fangquan, Lin, Junhao, Kang, Rui, Tang, Daolin, Liu, Jiao. 2024. Alkaliptosis induction counteracts paclitaxel-resistant ovarian cancer cells via ATP6V0D1-mediated ABCB1 inhibition. In Molecular carcinogenesis, 63, 1515-1527. doi:10.1002/mc.23741. https://pubmed.ncbi.nlm.nih.gov/38751020/
5. Jeong, Eutteum, Martina, José A, Contreras, Pablo S, Lee, Juhyung, Puertollano, Rosa. 2022. The FACT complex facilitates expression of lysosomal and antioxidant genes through binding to TFEB and TFE3. In Autophagy, 18, 2333-2349. doi:10.1080/15548627.2022.2029671. https://pubmed.ncbi.nlm.nih.gov/35230915/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen