C57BL/6JCya-Cabp4em1/Cya
Common Name
Cabp4-KO
Product ID
S-KO-19440
Backgroud
C57BL/6JCya
Strain ID
KOCMP-73660-Cabp4-B6J-VB
Status
When using this mouse strain in a publication, please cite “Cabp4-KO Mouse (Catalog S-KO-19440) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Cabp4-KO
Strain ID
KOCMP-73660-Cabp4-B6J-VB
Gene Name
Product ID
S-KO-19440
Gene Alias
2410038D05Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 19
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000025761
NCBI RefSeq
NM_144532
Target Region
Exon 2~3
Size of Effective Region
~1.1 kb
Overview of Gene Research
Cabp4, also known as calcium-binding protein 4, is a neuronal Ca2+-binding protein. It participates in many cellular processes by regulating the concentration of free Ca2+ ions, and is essential for the development and maintenance of the photoreceptor synapse in the retina [2,3]. It may also be involved in the regulation of voltage-gated Ca(v)1 Ca(2+) channels [4].
In Cabp4 knockout mice, the thickness of the outer plexiform layer and the number of photoreceptor terminals were reduced. The amplitude and sensitivity of the b-wave in double Cabp4/rod alpha-transducin knockout (Cabp4(-/-)Gnat1(-/-)) mice, which lack the rod-mediated component of electrophysiologic responses, were severely attenuated, indicating severe disruption of cone synaptic function [3]. In addition, a missense mutation (p.G155D) in Cabp4 in mice led to an imbalance in protein expression in brain regions and an increase in the frequency of micro-excitatory post-synaptic currents in layer II/III cortical pyramidal cells, suggesting its role in seizure onset [1].
In conclusion, Cabp4 is crucial for signal transmission from rods and cones to second-order neurons in the retina. The gene knockout mouse models have revealed its important role in retinal function and provided insights into its potential involvement in epilepsy-related mechanisms [1,3].
References：
1. Shi, Gang-An, Liang, Ming-Juan, Miao, Qin-Fei, Zhai, Qiong-Xiang, Chen, Zhi-Hong. 2024. CABP4 mutation in mice shows alteration in protein expression level and neuron discharge frequency. In Translational pediatrics, 13, 705-715. doi:10.21037/tp-23-484. https://pubmed.ncbi.nlm.nih.gov/38840676/
2. Guo, Yuxiong, Miao, Qinfei, Zhang, Yuxin, Zhai, Qiongxiang, Chen, Zhihong. . A novel missense creatine mutant of CaBP4, c.464G>A (p.G155D), associated with autosomal dominant nocturnal frontal lobe epilepsy (ADNFLE), reduces the expression of CaBP4. In Translational pediatrics, 11, 396-402. doi:10.21037/tp-22-54. https://pubmed.ncbi.nlm.nih.gov/35378956/
3. Maeda, Tadao, Lem, Janis, Palczewski, Krzysztof, Haeseleer, Françoise. . A critical role of CaBP4 in the cone synapse. In Investigative ophthalmology & visual science, 46, 4320-7. doi:. https://pubmed.ncbi.nlm.nih.gov/16249514/
4. Haeseleer, Françoise, Sokal, Izabela, Gregory, Frederick D, Lee, Amy. 2013. Protein phosphatase 2A dephosphorylates CaBP4 and regulates CaBP4 function. In Investigative ophthalmology & visual science, 54, 1214-26. doi:10.1167/iovs.12-11319. https://pubmed.ncbi.nlm.nih.gov/23341017/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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