C57BL/6JCya-Itgb6em1/Cya
Common Name:
Itgb6-KO
Product ID:
S-KO-19441
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Itgb6-KO
Strain ID
KOCMP-16420-Itgb6-B6J-VB
Gene Name
Product ID
S-KO-19441
Gene Alias
2210409C20Rik; 4831415H04Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Itgb6em1/Cya mice (Catalog S-KO-19441) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000059888
NCBI RefSeq
NM_021359
Target Region
Exon 3~4
Size of Effective Region
~1.8 kb
Detailed Document
Overview of Gene Research
Itgb6, encoding integrin β6, is a member of the integrin family. It frequently associates with integrin subunit αv to form integrin αvβ6, a transmembrane heterodimer involved in wound healing and disease pathogenesis like fibrosis and cancer [1,3,4]. Itgb6 determines αvβ6 expression and availability, and its study helps understand related molecular mechanisms. Gene knockout models can be valuable for functional studies of Itgb6.
In head and neck cancer, Itgb6 mediates differential responses to anti-CD276 treatment. It regulates the expression of CX3CL1, recruiting and activating PF4+ macrophages, and inhibition of the CX3CL1-CX3CR1 axis enhances sensitivity to anti-CD276 treatment [2]. In ITGB6-knockout HuCCT1 CCA cell lines, cell migration, invasion, wound-healing, and colony formation abilities were inhibited, and the cell cycle was dysregulated, with decreased PODXL2 expression [5]. In pancreatic cancer, ITGB6 knockdown in vitro and in vivo inhibited cancer cell proliferation and invasion [6].
In conclusion, Itgb6 is crucial in processes related to cancer. Through gene-knockout models, we've learned that Itgb6 plays roles in cancer cell proliferation, invasion, and response to immunotherapy. Understanding Itgb6's functions provides potential therapeutic targets for cancers and may help in developing more effective treatment strategies.
References：
1. Zhang, Zhe, Wang, Zheng, Liu, Tong, Yang, Yang, Zhang, Huan. 2023. Exploring the role of ITGB6: fibrosis, cancer, and other diseases. In Apoptosis : an international journal on programmed cell death, 29, 570-585. doi:10.1007/s10495-023-01921-6. https://pubmed.ncbi.nlm.nih.gov/38127283/
2. Zhang, Caihua, Li, Kang, Zhu, Hongzhang, Wang, Cheng, Chen, Demeng. 2024. ITGB6 modulates resistance to anti-CD276 therapy in head and neck cancer by promoting PF4+ macrophage infiltration. In Nature communications, 15, 7077. doi:10.1038/s41467-024-51096-0. https://pubmed.ncbi.nlm.nih.gov/39152118/
3. Meecham, Amelia, Marshall, John F. 2019. The ITGB6 gene: its role in experimental and clinical biology. In Gene, 763S, 100023. doi:10.1016/j.gene.2019.100023. https://pubmed.ncbi.nlm.nih.gov/34493369/
4. Meecham, Amelia, Marshall, John F. 2019. The ITGB6 gene: its role in experimental and clinical biology. In Gene: X, 5, 100023. doi:10.1016/j.gene.2019.100023. https://pubmed.ncbi.nlm.nih.gov/32550552/
5. Soejima, Yurie, Takeuchi, Miho, Miyamoto, Nao, Sawabe, Motoji, Fukusato, Toshio. 2021. ITGB6-Knockout Suppresses Cholangiocarcinoma Cell Migration and Invasion with Declining PODXL2 Expression. In International journal of molecular sciences, 22, . doi:10.3390/ijms22126303. https://pubmed.ncbi.nlm.nih.gov/34208313/
6. Zhong, Chao, Li, Zhi-Xi, Yang, Ling-Jing, Wang, Yu-Lan, Zhou, Qing. 2021. ITGB6 may promote proliferation and invasion in pancreatic cancer. In Archives of medical science : AMS, 20, 267-279. doi:10.5114/aoms/114039. https://pubmed.ncbi.nlm.nih.gov/38414469/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen