C57BL/6JCya-Gpa33em1/Cya
Common Name:
Gpa33-KO
Product ID:
S-KO-19450
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Gpa33-KO
Strain ID
KOCMP-59290-Gpa33-B6J-VC
Gene Name
Product ID
S-KO-19450
Gene Alias
2010310L10Rik; 2210401D16Rik; mA33
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Gpa33em1/Cya mice (Catalog S-KO-19450) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000060833
NCBI RefSeq
NM_021610
Target Region
Exon 3
Size of Effective Region
~2.5 kb
Detailed Document
Overview of Gene Research
Gpa33, also known as glycoprotein A33, is an Ig superfamily protein. It has been implicated in immune dysregulation, and its expression pattern can distinguish different cellular subsets, especially in the CD4+ T cell lineage [2]. In the immune system, it is expressed on fractions of B, dendritic, natural killer, and innate lymphoid cells, with prominent expression in the CD4+ T cell compartment [2].
Based on proteomic and transcriptomic approaches, GPA33 is expressed on a subset of human regulatory T (Treg) cells. It is acquired late during thymic Treg (tTreg) cell development but not expressed on TGF-β-induced Treg cells. GPA33 can identify Treg cells in human blood that lack the ability to produce effector cytokines. GPA33high Treg cells express the transcription factor Helios, can be stably expanded in vitro, and are suppressive, suggesting its potential use in isolating tTreg cells for adoptive cell therapy [1].
In conclusion, Gpa33 is important in immune-related cellular subset identification, especially in Treg cells. Its role in identifying stable tTreg cells provides a strategy for safer adoptive cell therapy, highlighting its significance in the field of immunotherapy [1].
References：
1. Opstelten, Rianne, de Kivit, Sander, Slot, Manon C, Cuadrado, Eloy, Amsen, Derk. 2020. GPA33: A Marker to Identify Stable Human Regulatory T Cells. In Journal of immunology (Baltimore, Md. : 1950), 204, 3139-3148. doi:10.4049/jimmunol.1901250. https://pubmed.ncbi.nlm.nih.gov/32366581/
2. Opstelten, Rianne, Suwandi, Jessica S, Slot, Manon C, Roep, Bart O, Amsen, Derk. 2021. GPA33 is expressed on multiple human blood cell types and distinguishes CD4+ central memory T cells with and without effector function. In European journal of immunology, 51, 1377-1389. doi:10.1002/eji.202048744. https://pubmed.ncbi.nlm.nih.gov/33728639/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen