C57BL/6JCya-Ccdc137em1/Cya
Common Name:
Ccdc137-KO
Product ID:
S-KO-19455
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Ccdc137-KO
Strain ID
KOCMP-67291-Ccdc137-B6J-VC
Gene Name
Product ID
S-KO-19455
Gene Alias
3110023B02Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
11
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ccdc137em1/Cya mice (Catalog S-KO-19455) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000058370
NCBI RefSeq
NM_152807
Target Region
Exon 3
Size of Effective Region
~1.8 kb
Detailed Document
Overview of Gene Research
CCDC137, a member of the coiled-coil domain containing (CCDC) family, is involved in multiple biological processes. It has been associated with important signaling pathways such as the β-catenin and AKT pathways [1,3]. In the context of diseases, it is significantly linked to cancer development and progression, playing a potential oncogenic role [1,3,4,5,7].
In hepatocellular carcinoma (HCC), elevated CCDC137 expression correlates with poor prognosis. It promotes HCC progression both in vitro and in vivo. Mechanistically, CCDC137 binds to LZTS2, a negative regulator of β-catenin, facilitating its K48-linked poly-ubiquitination via recruiting E3 ubiquitin ligase β-TrCP in the nucleus, thus activating the AKT and β-catenin pathways [1]. Also, CCDC137 binds to FOXM1, JTV1, LASP1, and FLOT2 mRNAs, increasing their cytoplasmic localization to enhance protein expressions, which in turn activates AKT signaling to promote HCC [3].
In colorectal cancer, CDK12 regulates the transcription of CCDC137, which is associated with hepatic metastasis [2]. Pan-cancer analysis shows that CCDC137 is over-expressed in various tumor types, associated with worse survival, and may contribute to an immunosuppressive tumor microenvironment [4].
In lung adenocarcinoma, CCDC137 was initially reported to be part of an oncogenic axis with CPSF1, though the related study was later retracted [7,8]. Additionally, in breast cancer, CCDC137 transcripts were found to contain a non-synonymous RNA variant unique to cancer tissue compared to adjacent normal tissue [9]. HIV-1 Vpr can deplete CCDC137, causing G2/M cell-cycle arrest and enhancing viral gene expression [6].
In conclusion, CCDC137 plays a crucial role in cancer-related biological processes, especially in HCC, colorectal cancer, and potentially in other cancer types. Its involvement in key signaling pathways makes it a potential therapeutic target. The studies on CCDC137, including those from in vitro and in vivo models, help in understanding its role in disease development, offering insights for developing targeted therapies against related cancers.
References：
1. Xu, Lei, Liu, Qiumeng, Liu, Hailing, Chen, Lin, Chen, Jin. 2024. Disrupting CCDC137-mediated LZTS2 and β-TrCP interaction in the nucleus inhibits hepatocellular carcinoma development via β-catenin and AKT. In Cell death and differentiation, 32, 134-148. doi:10.1038/s41418-024-01328-z. https://pubmed.ncbi.nlm.nih.gov/38918619/
2. Dai, Wei, Wu, Junhong, Peng, Xiaopeng, Zhou, Jingfeng, Liu, Shenglan. . CDK12 orchestrates super-enhancer-associated CCDC137 transcription to direct hepatic metastasis in colorectal cancer. In Clinical and translational medicine, 12, e1087. doi:10.1002/ctm2.1087. https://pubmed.ncbi.nlm.nih.gov/36254394/
3. Tao, Shuang, Xie, Shu-Juan, Diao, Li-Ting, Du, Bin, Xiao, Zhen-Dong. 2023. RNA-binding protein CCDC137 activates AKT signaling and promotes hepatocellular carcinoma through a novel non-canonical role of DGCR8 in mRNA localization. In Journal of experimental & clinical cancer research : CR, 42, 194. doi:10.1186/s13046-023-02749-3. https://pubmed.ncbi.nlm.nih.gov/37542342/
4. Guo, Lihao, Li, Boxin, Lu, Zhaohong, Xuan, Mei, Tang, Huanwen. 2021. CCDC137 Is a Prognostic Biomarker and Correlates With Immunosuppressive Tumor Microenvironment Based on Pan-Cancer Analysis. In Frontiers in molecular biosciences, 8, 674863. doi:10.3389/fmolb.2021.674863. https://pubmed.ncbi.nlm.nih.gov/34055889/
5. Bai, Lu, Yang, Zhao-Xu, Liu, Jian-Shan, Wang, De-Sheng, Yu, Heng-Chao. 2022. Prognostic Significance of CCDC137 Expression and Its Association with Immune Infiltration in Hepatocellular Carcinoma. In Disease markers, 2022, 5638675. doi:10.1155/2022/5638675. https://pubmed.ncbi.nlm.nih.gov/36061359/
6. Zhang, Fengwen, Bieniasz, Paul D. 2020. HIV-1 Vpr induces cell cycle arrest and enhances viral gene expression by depleting CCDC137. In eLife, 9, . doi:10.7554/eLife.55806. https://pubmed.ncbi.nlm.nih.gov/32538781/
7. Xudong, Xiang, Heng, Li, Benchao, Chen, Bao, Lei, Gaofeng, Li. 2024. Integrated RNA expression and alternative polyadenylation analysis identified CPSF1-CCDC137 oncogenic axis in lung adenocarcinoma. In Environmental toxicology, 39, 2405-2416. doi:10.1002/tox.24105. https://pubmed.ncbi.nlm.nih.gov/38174951/
8. . 2025. RETRACTION: Integrated RNA Expression and Alternative Polyadenylation Analysis Identified CPSF1-CCDC137 Oncogenic Axis in Lung Adenocarcinoma. In Environmental toxicology, 40, 711. doi:10.1002/tox.24478. https://pubmed.ncbi.nlm.nih.gov/39838867/
9. Hong, Ji Hyung, Ko, Yoon Ho, Kang, Keunsoo. 2018. RNA variant identification discrepancy among splice-aware alignment algorithms. In PloS one, 13, e0201822. doi:10.1371/journal.pone.0201822. https://pubmed.ncbi.nlm.nih.gov/30071094/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen