C57BL/6JCya-Zfp609em1/Cya
Common Name:
Zfp609-KO
Product ID:
S-KO-19457
Background:
C57BL/6JCya
Product Type
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Genotype
Sex
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Basic Information
Strain Name
Zfp609-KO
Strain ID
KOCMP-214812-Zfp609-B6J-VB
Gene Name
Product ID
S-KO-19457
Gene Alias
9830165N24; Znf609; mKIAA0295
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
9
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Zfp609em1/Cya mice (Catalog S-KO-19457) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000159109
NCBI RefSeq
NM_172536
Target Region
Exon 3
Size of Effective Region
~1.2 kb
Detailed Document
Overview of Gene Research
Zfp609, a zinc-finger protein, plays diverse and crucial roles in various biological processes. It is involved in pathways related to cell differentiation, neural development, and immune system regulation. Genetic models, especially KO/CKO mouse models, are valuable for studying its functions.
In muscle development, the circular RNA circZfp609, derived from the Zfp609 gene, suppresses myogenic differentiation by sponging miR-194-5p, which in turn sequesters its inhibition on BCLAF1 [1].
In the brain, Zfp609 interacts with Nipbl and the Integrator complex. Depletion of Zfp609 from cortical neural progenitors in vivo is detrimental to neuronal migration, indicating its importance in brain development [2].
In thymocytes, Zfp609 is necessary for Rag1 and Rag2 expression. Zfp608 represses Rag1 and Rag2 expression indirectly by repressing Zfp609 expression, highlighting the balance between Zfp608 and Zfp609 in regulating the adaptive immune system [3].
In conclusion, Zfp609 is essential for muscle differentiation, cortical neuron migration, and proper thymocyte development. Research using KO/CKO mouse models has provided insights into its functions in these biological processes, potentially offering new strategies for muscle-related disorders, neurological defects associated with Cornelia de Lange syndrome, and immune-related diseases.
References：
1. Wang, YanHong, Li, MengLu, Wang, YanHuan, Chen, Hong, Zhang, ChunLei. 2018. A Zfp609 circular RNA regulates myoblast differentiation by sponging miR-194-5p. In International journal of biological macromolecules, 121, 1308-1313. doi:10.1016/j.ijbiomac.2018.09.039. https://pubmed.ncbi.nlm.nih.gov/30201567/
2. van den Berg, Debbie L C, Azzarelli, Roberta, Oishi, Koji, Demmers, Jeroen A, Guillemot, François. 2016. Nipbl Interacts with Zfp609 and the Integrator Complex to Regulate Cortical Neuron Migration. In Neuron, 93, 348-361. doi:10.1016/j.neuron.2016.11.047. https://pubmed.ncbi.nlm.nih.gov/28041881/
3. Reed, N P, Henderson, M A, Oltz, E M, Aune, T M. 2012. Reciprocal regulation of Rag expression in thymocytes by the zinc-finger proteins, Zfp608 and Zfp609. In Genes and immunity, 14, 7-12. doi:10.1038/gene.2012.47. https://pubmed.ncbi.nlm.nih.gov/23076336/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen