C57BL/6JCya-Asic5em1/Cya
Common Name:
Asic5-KO
Product ID:
S-KO-19461
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Asic5-KO
Strain ID
KOCMP-58170-Asic5-B6J-VC
Gene Name
Product ID
S-KO-19461
Gene Alias
Accn5; Blinac; Inac
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
3
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Asic5em1/Cya mice (Catalog S-KO-19461) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000029641
NCBI RefSeq
NM_021370
Target Region
Exon 3
Size of Effective Region
~1.4 kb
Detailed Document
Overview of Gene Research
Asic5, also known as bile acid-sensitive ion channel (BASIC), is an orphan member of the acid-sensing ion channels (ASICs) family, which belongs to the ENaC/DEG family. ASICs are cationic channels activated by extracellular protons, widely distributed in the nervous system and play roles in various physiological and pathological processes [2,4,5,6].
In Asic5 knockout mouse models, deletion of Asic5 impaired motor performance. This is because Asic5 deletion decreased intrinsic excitability of type II unipolar brush cells (UBCs) in the vestibulocerebellum, reducing spontaneous action potential firing. Although there was partial compensation by suppressing certain K+ currents, the overall loss of Asic5 led to altered temporal processing of vestibular input, contributing to cerebellar ataxia [1,3].
In summary, Asic5 is important for the activity of type II UBCs in the vestibulocerebellum, influencing motor coordination and balance. The Asic5 knockout mouse model has revealed its role in cerebellar ataxia, providing insights into the underlying mechanisms of movement-related disorders [1,3].
References:
1. Kreko-Pierce, Tabita, Boiko, Nina, Harbidge, Donald G, Stockand, James D, Pugh, Jason R. 2020. Cerebellar Ataxia Caused by Type II Unipolar Brush Cell Dysfunction in the Asic5 Knockout Mouse. In Scientific reports, 10, 2168. doi:10.1038/s41598-020-58901-y. https://pubmed.ncbi.nlm.nih.gov/32034189/
2. Zhu, Yueqin, Hu, Xiaojie, Wang, Lili, Zuo, Longquan, Huang, Yan. . Recent Advances in Acid-sensitive Ion Channels in Central Nervous System Diseases. In Current pharmaceutical design, 28, 1406-1411. doi:10.2174/1381612828666220422084159. https://pubmed.ncbi.nlm.nih.gov/35466865/
3. Boiko, Nina, Kucher, Volodymyr, Wang, Bin, Stockand, James D. 2014. Restrictive expression of acid-sensing ion channel 5 (asic5) in unipolar brush cells of the vestibulocerebellum. In PloS one, 9, e91326. doi:10.1371/journal.pone.0091326. https://pubmed.ncbi.nlm.nih.gov/24663811/
4. Storozhuk, Maksim, Cherninskyi, Andrii, Maximyuk, Oleksandr, Isaev, Dmytro, Krishtal, Oleg. . Acid-Sensing Ion Channels: Focus on Physiological and Some Pathological Roles in the Brain. In Current neuropharmacology, 19, 1570-1589. doi:10.2174/1570159X19666210125151824. https://pubmed.ncbi.nlm.nih.gov/33550975/
5. Hanukoglu, Israel. 2016. ASIC and ENaC type sodium channels: conformational states and the structures of the ion selectivity filters. In The FEBS journal, 284, 525-545. doi:10.1111/febs.13840. https://pubmed.ncbi.nlm.nih.gov/27580245/
6. Vyvers, Anna, Schmidt, Axel, Wiemuth, Dominik, Gründer, Stefan. 2018. Screening of 109 neuropeptides on ASICs reveals no direct agonists and dynorphin A, YFMRFamide and endomorphin-1 as modulators. In Scientific reports, 8, 18000. doi:10.1038/s41598-018-36125-5. https://pubmed.ncbi.nlm.nih.gov/30573735/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen