C57BL/6JCya-Emp1em1/Cya
Common Name:
Emp1-KO
Product ID:
S-KO-19480
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Emp1-KO
Strain ID
KOCMP-13730-Emp1-B6J-VC
Gene Name
Product ID
S-KO-19480
Gene Alias
I-8-09; TMP
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
6
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Emp1em1/Cya mice (Catalog S-KO-19480) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000111907
NCBI RefSeq
NM_001288627
Target Region
Exon 3~4
Size of Effective Region
~1.2 kb
Detailed Document
Overview of Gene Research
Emp1, encoding epithelial membrane protein 1, belongs to the peripheral myelin protein 22-kDa (PMP22) gene family [2]. Its functions are diverse, with implications in cell proliferation, migration, metastasis, and tumorigenesis through various pathways like PI3K-AKT, RAS/RAF/MAPK, and PPARγ signaling [3,4,6].
In colorectal cancer, genetic ablation of EMP1high cells, marked by Emp1, prevented metastatic recurrence in a human-like mouse model of microsatellite-stable CRC. Residual EMP1+ high-relapse cells (HRCs) in mouse livers after primary CRC surgery could give rise to multiple cell types and cause metastatic disease [1].
In osteosarcoma, knockdown of Emp1 inhibited migratory and invasive abilities of OS cells, as Emp1 was upregulated in OS tissues and promoted the malignant progression through the IRX2/MMP9 axis [5].
In gliomas, silencing Emp1 inhibited cell invasion and proliferation by inhibiting the PI3K-AKT signaling pathway and regulated stemness by affecting CD44 expression [6].
In bladder cancer, loss of Emp1 promoted migration and resistance to ferroptosis via activation of PPARγ signaling [3].
In conclusion, Emp1 plays a crucial role in the progression of multiple cancers including colorectal, osteosarcoma, glioma, and bladder cancer. Gene-knockout models in mice have been instrumental in revealing these functions, highlighting its potential as a therapeutic target for preventing cancer metastasis and relapse.
References：
1. Cañellas-Socias, Adrià, Cortina, Carme, Hernando-Momblona, Xavier, Attolini, Camille Stephan-Otto, Batlle, Eduard. 2022. Metastatic recurrence in colorectal cancer arises from residual EMP1+ cells. In Nature, 611, 603-613. doi:10.1038/s41586-022-05402-9. https://pubmed.ncbi.nlm.nih.gov/36352230/
2. Wang, Yi-Wen, Cheng, Hong-Ling, Ding, Ya-Rou, Chou, Lien-Hsuan, Chow, Nan-Haw. 2017. EMP1, EMP 2, and EMP3 as novel therapeutic targets in human cancer. In Biochimica et biophysica acta. Reviews on cancer, 1868, 199-211. doi:10.1016/j.bbcan.2017.04.004. https://pubmed.ncbi.nlm.nih.gov/28408326/
3. Liu, Sha, Shi, Jiazhong, Wang, Liwei, Chen, Zhiwen, Yang, Jin. 2022. Loss of EMP1 promotes the metastasis of human bladder cancer cells by promoting migration and conferring resistance to ferroptosis through activation of PPAR gamma signaling. In Free radical biology & medicine, 189, 42-57. doi:10.1016/j.freeradbiomed.2022.06.247. https://pubmed.ncbi.nlm.nih.gov/35850179/
4. Han, Ying, Gong, Jin, Pan, Min, Cai, Wenqin, Peng, Xiane. . EMP1 knockdown mitigated high glucose-induced pyroptosis and oxidative stress in rat H9c2 cardiomyocytes by inhibiting the RAS/RAF/MAPK signaling pathway. In Journal of biochemical and molecular toxicology, 38, e70002. doi:10.1002/jbt.70002. https://pubmed.ncbi.nlm.nih.gov/39415664/
5. Wang, Mingfa, Liu, Tianyu, Hu, Xiaowei, Liu, Jingmin, Wang, Xiaoge. . EMP1 promotes the malignant progression of osteosarcoma through the IRX2/MMP9 axis. In Panminerva medica, 62, 150-154. doi:10.23736/S0031-0808.20.03913-0. https://pubmed.ncbi.nlm.nih.gov/32716150/
6. Wang, Junxiang, Li, Xuetao, Wu, Haibin, Deng, Zhitong, Zhou, Youxin. 2019. EMP1 regulates cell proliferation, migration, and stemness in gliomas through PI3K-AKT signaling and CD44. In Journal of cellular biochemistry, 120, 17142-17150. doi:10.1002/jcb.28974. https://pubmed.ncbi.nlm.nih.gov/31111534/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen