C57BL/6JCya-Gstk1em1/Cya
Common Name:
Gstk1-KO
Product ID:
S-KO-19487
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Gstk1-KO
Strain ID
KOCMP-76263-Gstk1-B6J-VC
Gene Name
Product ID
S-KO-19487
Gene Alias
0610025I19Rik; DsbA-L
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
6
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Gstk1em1/Cya mice (Catalog S-KO-19487) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000031897
NCBI RefSeq
NM_029555
Target Region
Exon 2~3
Size of Effective Region
~0.9 kb
Detailed Document
Overview of Gene Research
Gstk1, also known as glutathione S-transferase kappa 1, is a key enzyme in multiple biological processes. It is involved in maintaining peroxisomal redox homeostasis, with its glutathione-dependent disulfide bond oxidoreductase activity playing a role in regulating the intra-peroxisomal redox state [1]. It is also associated with energy production, lipid metabolism, and adiponectin secretion and multimerization [3,5]. Additionally, it may impact insulin resistance and related metabolic diseases, as well as sperm quality [3,4].
In five mouse HCM models of different etiologies, Gstk1 was significantly downregulated. Gene-gene functional interaction network analysis suggested correlative expression of Gstk1 with other genes. Knocking out gstk1 in zebrafish increased the expression of certain sarcomere genes and affected cardiac function, indicating that Gstk1 downregulation might be a common mechanism underlying HCM, possibly through increasing oxidative stress [2]. In HEK-293 cells, ablation of Gstk1 did not change the basal intraperoxisomal redox state but significantly extended the recovery period of the peroxisomal glutathione redox sensor upon treatment with thiol-specific oxidants, demonstrating its role in peroxisomal glutathione regulation [1].
In conclusion, Gstk1 is crucial for maintaining peroxisomal redox balance, energy and lipid metabolism, and adiponectin-related processes. Its downregulation in HCM models suggests a potential role in the molecular pathogenesis of HCM. Studies using gene-knockout models in mice and zebrafish have provided valuable insights into its functions and implications in disease, helping to understand the underlying mechanisms and potentially guiding the development of new therapeutic strategies for related diseases.
References:
1. Costa, Cláudio F, Lismont, Celien, Chornyi, Serhii, Waterham, Hans R, Fransen, Marc. 2023. Functional Analysis of GSTK1 in Peroxisomal Redox Homeostasis in HEK-293 Cells. In Antioxidants (Basel, Switzerland), 12, . doi:10.3390/antiox12061236. https://pubmed.ncbi.nlm.nih.gov/37371965/
2. Sasagawa, Shota, Nishimura, Yuhei, Okabe, Shiko, Ito, Masaaki, Tanaka, Toshio. 2016. Downregulation of GSTK1 Is a Common Mechanism Underlying Hypertrophic Cardiomyopathy. In Frontiers in pharmacology, 7, 162. doi:10.3389/fphar.2016.00162. https://pubmed.ncbi.nlm.nih.gov/27378925/
3. Hu, Chun, Sun, Lin. . [Advance in glutathione S-transferase kappa 1]. In Zhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences, 42, 468-475. doi:10.11817/j.issn.1672-7347.2017.04.017. https://pubmed.ncbi.nlm.nih.gov/28490708/
4. Zeng, Fanwen, Li, Chengde, Huang, Jianhao, Wei, Hengxi, Zhang, Shouquan. 2021. Glutathione S-transferase kappa 1 is positively related with sperm quality of porcine sperm. In Molecular reproduction and development, 89, 104-112. doi:10.1002/mrd.23551. https://pubmed.ncbi.nlm.nih.gov/34888969/
5. Morel, Fabrice, Aninat, Caroline. 2011. The glutathione transferase kappa family. In Drug metabolism reviews, 43, 281-91. doi:10.3109/03602532.2011.556122. https://pubmed.ncbi.nlm.nih.gov/21428694/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen