C57BL/6JCya-Dact3em1/Cya
Common Name:
Dact3-KO
Product ID:
S-KO-19489
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Dact3-KO
Strain ID
KOCMP-629378-Dact3-B6J-VB
Gene Name
Product ID
S-KO-19489
Gene Alias
EG666671; naa-3
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
7
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Dact3em1/Cya mice (Catalog S-KO-19489) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000108493
NCBI RefSeq
NM_001081655
Target Region
Exon 2~3
Size of Effective Region
~2.1 kb
Detailed Document
Overview of Gene Research
Dact3, short for Dapper antagonist of catenin-3, is a protein-coding gene. It is a negative regulator of the Wnt/β-catenin signaling pathway, which is crucial in many biological processes such as cell proliferation, differentiation, and apoptosis [1,2,3,4,6]. Dysregulation of this pathway is often associated with various diseases, especially cancers.
In acute myeloid leukemia (AML), low Dact3 levels are associated with shorter survival rates. Upregulation of Dact3 represses cellular proliferation, promotes cell cycle arrest and apoptosis of AML cells by decreasing levels of Dishevelled2 (DVL2), phospho-glycogen synthase kinase-3β (GSK-3β), and active β-catenin, thus suppressing Wnt/β-catenin-mediated transcriptional activity [1].
In non-small cell lung cancer (NSCLC), Dact3 expression is reduced, and its reduction is correlated with lymph node metastasis and poor prognosis. Dact3 may inhibit the malignant phenotype of NSCLC through downregulating c-Myb [3].
In glioma, a decrease in Dact3 expression is observed in tissues. Overexpression of Dact3 suppresses the proliferation, invasion, and migration of glioma cells, and increases cell adhesion by impeding the expression of notch 1 intracellular domain (NICD) and translocating into the nucleus by downregulating β-catenin, thereby suppressing Notch1 signaling [5].
In conclusion, Dact3 plays a significant tumor-suppressive role in multiple cancers, mainly through its regulation of the Wnt/β-catenin signaling pathway. Research on Dact3 knockout or conditional knockout mouse models could potentially further elucidate its role in these disease conditions, providing insights into cancer pathogenesis and potential therapeutic strategies.
References:
1. Gao, Qiuying, Hou, Limin, Wang, Hui, Xun, Liru. 2022. DACT3 has a tumor-inhibiting role in acute myeloid leukemia via the suppression of Wnt/β-catenin signaling by DVL2. In Journal of biochemical and molecular toxicology, 36, e23014. doi:10.1002/jbt.23014. https://pubmed.ncbi.nlm.nih.gov/35187752/
2. Kim, Soo Jin, Kim, Suntae, Choi, Yong June, Kim, U Ji, Kang, Keon Wook. 2022. CKD-581 Downregulates Wnt/β-Catenin Pathway by DACT3 Induction in Hematologic Malignancy. In Biomolecules & therapeutics, 30, 435-446. doi:10.4062/biomolther.2022.022. https://pubmed.ncbi.nlm.nih.gov/35794797/
3. Zhao, Huanyu, Yang, Lianhe, Han, Yang, Wang, Enhua, Wu, Guangping. 2017. Dact3 inhibits the malignant phenotype of non-small cell lung cancer through downregulation of c-Myb. In International journal of clinical and experimental pathology, 10, 11580-11587. doi:. https://pubmed.ncbi.nlm.nih.gov/31966514/
4. Jiang, Xia, Tan, Jing, Li, Jingsong, Cheyette, Benjamin N R, Yu, Qiang. . DACT3 is an epigenetic regulator of Wnt/beta-catenin signaling in colorectal cancer and is a therapeutic target of histone modifications. In Cancer cell, 13, 529-41. doi:10.1016/j.ccr.2008.04.019. https://pubmed.ncbi.nlm.nih.gov/18538736/
5. Yue, Jianhe, Zhang, Jiqin, Huan, Renzheng, Tan, Ying, Cheng, Yuan. 2023. Dishevelled-associated antagonist of β-catenin homolog 3 (DACT3) suppresses glioma progression though Notch1 signaling pathway in β-catenin-dependent manner. In Heliyon, 10, e23511. doi:10.1016/j.heliyon.2023.e23511. https://pubmed.ncbi.nlm.nih.gov/38230242/
6. Ren, Yanli, Chen, Yujie, Liang, Xue, Pan, Wenting, Yang, Ming. 2017. MiRNA-638 promotes autophagy and malignant phenotypes of cancer cells via directly suppressing DACT3. In Cancer letters, 390, 126-136. doi:10.1016/j.canlet.2017.01.009. https://pubmed.ncbi.nlm.nih.gov/28108314/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen