C57BL/6JCya-Asb1em1/Cya
Common Name:
Asb1-KO
Product ID:
S-KO-19497
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Asb1-KO
Strain ID
KOCMP-65247-Asb1-B6J-VC
Gene Name
Product ID
S-KO-19497
Gene Alias
1700029O08Rik; 1700054C17Rik; mKIAA1146
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
1
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Asb1em1/Cya mice (Catalog S-KO-19497) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000086843
NCBI RefSeq
NM_001039126
Target Region
Exon 4
Size of Effective Region
~1.4 kb
Detailed Document
Overview of Gene Research
Asb1, short for Ankyrin Repeat and SOCS Box Containing 1, is the substrate recognition subunit of a ubiquitin ligase. It is involved in ubiquitination processes, which play crucial roles in regulating protein stability and various cellular functions. Ubiquitination can target proteins for degradation, thus affecting multiple biological pathways. Asb1's functions are important in different biological contexts, and genetic models like knockout mice are valuable for studying its roles [2].
In prostate cancer, Asb1 expression is significantly decreased in cancer tissues, correlating with poor patient prognosis. Silencing Asb1 enhances prostate cancer cell proliferation, clonogenicity, and migration, while overexpression has the opposite effects. Asb1 interacts with CHCHD3, promoting its degradation via K48-linked ubiquitination, and inhibits prostate cancer cells through the CHCHD3/reactive oxygen species (ROS) pathway [1].
In male infertility, Asb1-knockout (Asb1-KO) mice exhibit severe fertility impairment, characterized by oligoasthenoteratozoospermia, excessive oxidative stress, decreased hydrogen sulfide (H2S) levels in testes, and severe sperm DNA damage. ASB1 interacts with ELOB to enhance the K48-linked ubiquitination of sulfide-quinone oxidoreductase (SQOR) on residues K207 and K344, triggering its proteasomal degradation, which is crucial for H2S homeostasis and redox balance [2].
In ischaemic cardiomyopathy, a hypermethylated profile in the Asb1 gene is related to left ventricular ejection fraction, stroke volume, and end-systolic and diastolic left ventricular diameters, and Asb1 shows down-regulation in messenger RNA levels [3].
In conclusion, Asb1 is essential in multiple biological processes. Through model-based research, it has been shown to act as a tumor suppressor in prostate cancer, play a vital role in spermiogenesis related to male infertility, and be associated with left ventricular performance in ischaemic cardiomyopathy. These findings from Asb1 KO mouse models contribute to understanding the underlying mechanisms in these disease areas.
References：
1. Zhao, Chunchun, Xu, Zhen, Que, Hongliang, Tan, Ruoyun, Fan, Caibin. 2024. ASB1 inhibits prostate cancer progression by destabilizing CHCHD3 via K48-linked ubiquitination. In American journal of cancer research, 14, 3404-3418. doi:10.62347/FEIZ7492. https://pubmed.ncbi.nlm.nih.gov/39113857/
2. Lv, Jinxing, Wu, Tiantian, Xue, Jiajia, Huang, Xiaoyan, Zheng, Bo. 2024. ASB1 engages with ELOB to facilitate SQOR ubiquitination and H2S homeostasis during spermiogenesis. In Redox biology, 79, 103484. doi:10.1016/j.redox.2024.103484. https://pubmed.ncbi.nlm.nih.gov/39733518/
3. Ortega, Ana, Tarazón, Estefanía, Gil-Cayuela, Carolina, Roselló-Lletí, Esther, Rivera, Miguel. 2018. ASB1 differential methylation in ischaemic cardiomyopathy: relationship with left ventricular performance in end-stage heart failure patients. In ESC heart failure, 5, 732-737. doi:10.1002/ehf2.12289. https://pubmed.ncbi.nlm.nih.gov/29667349/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen