C57BL/6JCya-Cyp2b19em1/Cya
Common Name:
Cyp2b19-KO
Product ID:
S-KO-19499
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Cyp2b19-KO
Strain ID
KOCMP-13090-Cyp2b19-B6J-VC
Gene Name
Product ID
S-KO-19499
Gene Alias
CYPIIB19
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
7
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cyp2b19em1/Cya mice (Catalog S-KO-19499) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000077855
NCBI RefSeq
NM_007814
Target Region
Exon 2~3
Size of Effective Region
~2.5 kb
Detailed Document
Overview of Gene Research
Cyp2b19 is a cytochrome P450 enzyme with arachidonic acid monooxygenase activity. It is highly expressed in the outer, differentiated cell layers of mouse epidermis and is a specific cellular marker of late differentiation in skin keratinocytes. It is involved in arachidonic acid metabolism, generating epoxyeicosatrienoic acids (EETs) and hydroxyeicosatetraenoic acids (HETEs), which are implicated in regulating epidermal cornification [1,2].
Research on primary cultures of mouse epidermal keratinocytes showed that differentiation upregulated Cyp2b19 mRNA levels, immunoreactivity, and the cellular levels of its product 11,12-EET [1]. Recombinant Cyp2b19 metabolizes arachidonic acid to generate EETs and HETEs in a stereoselective manner, and the EETs produced are endogenous constituents of murine epidermis, suggesting it might be the primary source of these EETs [2]. Also, in a study on type 2 diabetes mellitus-induced gastroenteropathy in db/db mice, Cyp2b19 was identified as one of the major proteins associated with T2DM-induced gastrointestinal injury, indicating its possible role in this disease-related process [3].
In conclusion, Cyp2b19 plays a crucial role in epidermal cornification through its involvement in arachidonic acid metabolism to produce EETs. Its association with T2DM-induced gastrointestinal injury also implies its potential significance in diabetes-related gastrointestinal pathophysiology. Mouse models, such as the db/db mice, have been valuable in uncovering these functions and disease-related associations of Cyp2b19.
References:
1. Du, Liping, Yermalitsky, Valery, Ladd, Patricia A, Mernaugh, Raymond, Keeney, Diane S. . Evidence that cytochrome P450 CYP2B19 is the major source of epoxyeicosatrienoic acids in mouse skin. In Archives of biochemistry and biophysics, 435, 125-33. doi:. https://pubmed.ncbi.nlm.nih.gov/15680914/
2. Keeney, D S, Skinner, C, Travers, J B, King, L E, Waterman, M R. . Differentiating keratinocytes express a novel cytochrome P450 enzyme, CYP2B19, having arachidonate monooxygenase activity. In The Journal of biological chemistry, 273, 32071-9. doi:. https://pubmed.ncbi.nlm.nih.gov/9822682/
3. Zhang, Yuxin, Zhang, Yanjiao, Yin, Ruiyang, Yao, Yiqi, Tian, Jiaxing. 2024. Multi-omics characterization of type 2 diabetes mellitus-induced gastroenteropathy in the db/db mouse model. In Frontiers in cell and developmental biology, 12, 1417255. doi:10.3389/fcell.2024.1417255. https://pubmed.ncbi.nlm.nih.gov/39211388/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen