C57BL/6JCya-Lrrc19em1/Cya
Common Name:
Lrrc19-KO
Product ID:
S-KO-19504
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Lrrc19-KO
Strain ID
KOCMP-100061-Lrrc19-B6J-VB
Gene Name
Product ID
S-KO-19504
Gene Alias
9130022A01Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
4
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Lrrc19em1/Cya mice (Catalog S-KO-19504) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000107101
NCBI RefSeq
NM_001356281
Target Region
Exon 4
Size of Effective Region
~1.4 kb
Detailed Document
Overview of Gene Research
Lrrc19, leucine-rich repeat-containing protein 19, is a transmembrane protein belonging to the LRR protein family. Its central core has four LRR repeating modules and a casein kinase (CK2) phosphorylation site in the cytoplasmic domain. It activates NF-κB, induces production of pro-inflammatory cytokines, and is involved in innate immune responses [3]. It also has a role in maintaining the integrity of the gut epithelial barrier and in preventing uropathogenic bacteria infection in the kidney [1,2].
In Lrrc19 knockout (KO) mice, the gut epithelial barrier is more intact, with a tighter junction and narrower gaps in colon epithelium cells. There are lower levels of serum lipopolysaccharide and 4 kDa-fluorescein isothiocyanate-dextran after gavage, indicating reduced gut permeability. This is due to Lrrc19 degrading PKC-ζ and PKCι/λ, which reduces the expression of ZO-1, ZO-3, and occludin [1]. Lrrc19-deficient mice are also more susceptible to uropathogenic Escherichia coli (UPEC) infection, as recognition of UPEC by Lrrc19 in normal mice induces cytokine, chemokine, and antimicrobial substance production through TRAF2-and TRAF6-mediated NF-κB and MAPK signalling pathways [2]. In maternal high-fat diet (MHFD) studies, Lrrc19 deletion alleviated the disruption of the intestinal mucus barrier in offspring, with gut microbiota and metabolite imbalances restored [4].
In conclusion, Lrrc19 plays essential roles in the innate immune response, gut epithelial barrier function, and prevention of uropathogenic bacteria infection. The use of Lrrc19 KO mouse models has provided valuable insights into its role in maintaining gut and kidney homeostasis, as well as in understanding how diet-related factors like MHFD can impact these functions through Lrrc19-mediated mechanisms.
References:
1. Su, Xiaomin, Wei, Jianmei, Qi, Houbao, Zhang, Chunze, Yang, Rongcun. . LRRC19 Promotes Permeability of the Gut Epithelial Barrier Through Degrading PKC-ζ and PKCι/λ to Reduce Expression of ZO1, ZO3, and Occludin. In Inflammatory bowel diseases, 27, 1302-1315. doi:10.1093/ibd/izaa354. https://pubmed.ncbi.nlm.nih.gov/33501933/
2. Su, Xiaomin, Min, Siping, Cao, Shuisong, Che, Yongzhe, Yang, Rongcun. 2014. LRRC19 expressed in the kidney induces TRAF2/6-mediated signals to prevent infection by uropathogenic bacteria. In Nature communications, 5, 4434. doi:10.1038/ncomms5434. https://pubmed.ncbi.nlm.nih.gov/25026888/
3. Chai, Limin, Dai, Lingyun, Che, Yongzhe, Zhang, Zhujun, Yang, Rongcun. 2009. LRRC19, a novel member of the leucine-rich repeat protein family, activates NF-kappaB and induces expression of proinflammatory cytokines. In Biochemical and biophysical research communications, 388, 543-8. doi:10.1016/j.bbrc.2009.08.043. https://pubmed.ncbi.nlm.nih.gov/19679103/
4. Sun, Yue, Huang, Shumin, Li, Mengfan, Su, Xiaomin, Cao, Hailong. 2025. Maternal high-fat diet disrupts intestinal mucus barrier of offspring by regulating gut immune receptor LRRC19. In Communications biology, 8, 420. doi:10.1038/s42003-025-07836-z. https://pubmed.ncbi.nlm.nih.gov/40075219/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen