C57BL/6JCya-Rhagem1/Cya
Common Name:
Rhag-KO
Product ID:
S-KO-19510
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Rhag-KO
Strain ID
KOCMP-19743-Rhag-B6J-VC
Gene Name
Product ID
S-KO-19510
Gene Alias
CD241; Rh50; Rh50A
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
17
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Rhagem1/Cya mice (Catalog S-KO-19510) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000024721
NCBI RefSeq
NM_011269
Target Region
Exon 2~3
Size of Effective Region
~2.4 kb
Detailed Document
Overview of Gene Research
Rhag, the gene encoding Rh-associated glycoprotein, is essential for the assembly of the Rh protein complex in the erythrocyte membrane and for the expression of Rh antigens [3]. It is also implicated in maintaining the structure and stability of erythrocytes [2]. Rhag may play a role in gas transport, with studies suggesting its major contribution to the NH₃ conductance of erythrocytes [1].
Mice with inactivated Rhag genes (Rhag-/ -) have a total absence of Rhag and Rh proteins [4]. These knockout mouse models help delineate the antigenic, biochemical and functional abnormalities of red cells, and the impact on hematological parameters and erythropoiesis [4]. In humans, single missense mutations in RhAG can induce spherocytosis and deficiencies in Rh complex formation [2]. A novel missense mutation was found to incompletely disrupt Rh antigen expression and selectively knock out RhD antigen expression [2]. Also, certain mutations in RhAG underlie the extremely rare Rhnull phenotype, characterized by the absence of all Rh antigens on erythrocytes [5].
In conclusion, Rhag is crucial for Rh antigen expression and erythrocyte stability. Research using gene-knockout mouse models has revealed its role in antigen expression, red cell function, and in relation to rare blood phenotypes like Rhnull. Understanding Rhag contributes to a better comprehension of erythrocyte-related biological processes and associated disorders.
References：
1. Ripoche, P, Goossens, D, Devuyst, O, Verkman, A S, Cartron, J-P. 2006. Role of RhAG and AQP1 in NH3 and CO2 gas transport in red cell ghosts: a stopped-flow analysis. In Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine, 13, 117-22. doi:. https://pubmed.ncbi.nlm.nih.gov/16574458/
2. Mu, S, Cui, Y, Wang, W, Zhu, O, Zhu, D. 2018. A RHAG point mutation selectively disrupts Rh antigen expression. In Transfusion medicine (Oxford, England), 29, 121-127. doi:10.1111/tme.12519. https://pubmed.ncbi.nlm.nih.gov/29508504/
3. Avent, N D, Reid, M E. . The Rh blood group system: a review. In Blood, 95, 375-87. doi:. https://pubmed.ncbi.nlm.nih.gov/10627438/
4. Goossens, D, Bony, V, Gane, P, Colin, Y, Cartron, J-P. 2006. Generation of mice with inactivated Rh or Rhag genes. In Transfusion clinique et biologique : journal de la Societe francaise de transfusion sanguine, 13, 164-6. doi:. https://pubmed.ncbi.nlm.nih.gov/16581281/
5. Hellberg, Åsa, Arsenovic, Mirjana Grujic, Sørvoll, Ingvild Hausberg, Olsson, Martin L, Storry, Jill R. 2023. A novel nonsense variant in RHAG underlies a Nordic Rhnull phenotype. In Vox sanguinis, 118, 690-694. doi:10.1111/vox.13478. https://pubmed.ncbi.nlm.nih.gov/37265146/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen