C57BL/6JCya-Prdm13em1/Cya
Common Name:
Prdm13-KO
Product ID:
S-KO-19515
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Prdm13-KO
Strain ID
KOCMP-230025-Prdm13-B6J-VC
Gene Name
Product ID
S-KO-19515
Gene Alias
--
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
4
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Prdm13em1/Cya mice (Catalog S-KO-19515) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000095141
NCBI RefSeq
NM_001080771
Target Region
Exon 2~3
Size of Effective Region
~2.3 kb
Detailed Document
Overview of Gene Research
PRDM13, a transcriptional repressor, is a crucial gene involved in neuronal subtype specification. It functions downstream of the transcription factor PTF1A, controlling GABAergic cell fate in the spinal cord and retina, and neurogenesis in the hypothalamus [2,3,4,6,7,8]. It also plays a role in maintaining the balance between inhibitory and excitatory neurons in the dorsal spinal cord [6,7].
In zebrafish, loss-of-function of prdm13 leads to a reduction in Purkinje cells numbers and absence of the inferior olive nuclei, and in mouse models, Prdm13-deficient mice show cerebellar hypoplasia, delayed pubertal onset, and abnormal visual sensitivities [1,2,4]. In the retina, Prdm13-knockout mice have a loss of Ebf3+ amacrine cells and altered calretinin expression [3]. In the dorsomedial hypothalamus (DMH) of mice, Prdm13+ neurons are involved in sleep control, as DMH-specific Prdm13-knockout mice recapitulate age-associated sleep alterations [5].
In conclusion, Prdm13 is essential for the development and function of the nervous system, including the cerebellum, hypothalamus, and retina. The study of Prdm13 using gene-knockout mouse models has provided insights into its role in pontocerebellar hypoplasias, congenital hypogonadotropic hypogonadism, and sleep-wake regulation [1,2,5].
References:
1. Coolen, Marion, Altin, Nami, Rajamani, Karthyayani, Moutton, Sébastien, Cantagrel, Vincent. 2022. Recessive PRDM13 mutations cause fatal perinatal brainstem dysfunction with cerebellar hypoplasia and disrupt Purkinje cell differentiation. In American journal of human genetics, 109, 909-927. doi:10.1016/j.ajhg.2022.03.010. https://pubmed.ncbi.nlm.nih.gov/35390279/
2. Whittaker, Danielle E, Oleari, Roberto, Gregory, Louise C, Basson, M Albert, Dattani, Mehul T. . A recessive PRDM13 mutation results in congenital hypogonadotropic hypogonadism and cerebellar hypoplasia. In The Journal of clinical investigation, 131, . doi:10.1172/JCI141587. https://pubmed.ncbi.nlm.nih.gov/34730112/
3. Goodson, Noah B, Nahreini, Jhenya, Randazzo, Grace, Johnson, Jane E, Brzezinski, Joseph A. 2017. Prdm13 is required for Ebf3+ amacrine cell formation in the retina. In Developmental biology, 434, 149-163. doi:10.1016/j.ydbio.2017.12.003. https://pubmed.ncbi.nlm.nih.gov/29258872/
4. Watanabe, Satoshi, Sanuki, Rikako, Sugita, Yuko, Ohsuga, Mizuki, Furukawa, Takahisa. . Prdm13 regulates subtype specification of retinal amacrine interneurons and modulates visual sensitivity. In The Journal of neuroscience : the official journal of the Society for Neuroscience, 35, 8004-20. doi:10.1523/JNEUROSCI.0089-15.2015. https://pubmed.ncbi.nlm.nih.gov/25995483/
5. Tsuji, Shogo, Brace, Cynthia S, Yao, Ruiqing, Imai, Shin-Ichiro, Satoh, Akiko. 2023. Sleep-wake patterns are altered with age, Prdm13 signaling in the DMH, and diet restriction in mice. In Life science alliance, 6, . doi:10.26508/lsa.202301992. https://pubmed.ncbi.nlm.nih.gov/37045472/
6. Mona, Bishakha, Uruena, Ana, Kollipara, Rahul K, Chang, Joshua C, Johnson, Jane E. 2017. Repression by PRDM13 is critical for generating precision in neuronal identity. In eLife, 6, . doi:10.7554/eLife.25787. https://pubmed.ncbi.nlm.nih.gov/28850031/
7. Chang, Joshua C, Meredith, David M, Mayer, Paul R, Ou, Yi-Hung, Johnson, Jane E. . Prdm13 mediates the balance of inhibitory and excitatory neurons in somatosensory circuits. In Developmental cell, 25, 182-95. doi:10.1016/j.devcel.2013.02.015. https://pubmed.ncbi.nlm.nih.gov/23639443/
8. Bessodes, Nathalie, Parain, Karine, Bronchain, Odile, Bellefroid, Eric J, Perron, Muriel. 2017. Prdm13 forms a feedback loop with Ptf1a and is required for glycinergic amacrine cell genesis in the Xenopus Retina. In Neural development, 12, 16. doi:10.1186/s13064-017-0093-2. https://pubmed.ncbi.nlm.nih.gov/28863786/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen