C57BL/6JCya-Chodlem1/Cya
Common Name:
Chodl-KO
Product ID:
S-KO-19518
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Chodl-KO
Strain ID
KOCMP-246048-Chodl-B6J-VB
Gene Name
Product ID
S-KO-19518
Gene Alias
3110074E07Rik; MT75; PRED12
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
16
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Chodlem1/Cya mice (Catalog S-KO-19518) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000023568
NCBI RefSeq
NM_139134
Target Region
Exon 2~3
Size of Effective Region
~1.3 kb
Detailed Document
Overview of Gene Research
Chodl, also known as chondrolectin, is a gene encoding a type I transmembrane protein belonging to the C-type lectin family. It is predominantly expressed in muscle cells such as skeletal muscle, and is also found in tissues like testis, brain, and lung [5]. Chodl has been implicated in multiple biological processes. In the nervous system, it is involved in motor axon extension and neuromuscular junction formation [4].
In disease-related studies, Chodl shows differential expression patterns. In colorectal cancer, promoter hypermethylation of CHODL leads to its downregulation, promoting carcinogenesis, and is a poor prognostic factor for early-stage patients [1]. In hepatocellular carcinoma, CHODL expression is decreased in clinical samples and cell lines, yet overexpression in SMMC7721 cells increases cell migration and invasion [2]. In high-grade serous ovarian cancer, elevated CHODL expression is seen in metastasis tissues, and knockdown experiments show its role in reducing cell migration and invasion abilities [3]. In lung cancer, strong CHODL protein positivity is associated with shorter patient survival, and targeting CHODL might be a strategy for anticancer drug development [6].
In conclusion, Chodl plays essential roles in processes like neuromuscular junction formation. Its abnormal regulation is closely associated with multiple cancers, including colorectal, hepatocellular, ovarian, and lung cancers. Understanding Chodl through functional studies, especially those using gene knockout models as implied in some of these disease-related findings, can provide insights into disease mechanisms and potential therapeutic strategies.
References:
1. Zhang, Xinyue, Wu, Kaiming, Huang, Yuhua, Li, Xiaoxing, Zhang, Ning. 2020. Promoter Hypermethylation of CHODL Contributes to Carcinogenesis and Indicates Poor Survival in Patients with Early-stage Colorectal Cancer. In Journal of Cancer, 11, 2874-2886. doi:10.7150/jca.38815. https://pubmed.ncbi.nlm.nih.gov/32226505/
2. Huang, Zejian, Zhang, Ning, Li, Wenda, Zhang, Lei, Chen, Yajin. 2016. Expression of CHODL in hepatocellular carcinoma affects invasion and migration of liver cancer cells. In Oncology letters, 13, 715-721. doi:10.3892/ol.2016.5466. https://pubmed.ncbi.nlm.nih.gov/28356950/
3. Luo, Haixia, Wang, Kunyu, Li, Bin. 2024. Integrating single-cell and spatial transcriptomic analysis to unveil heterogeneity in high-grade serous ovarian cancer. In Frontiers in immunology, 15, 1420847. doi:10.3389/fimmu.2024.1420847. https://pubmed.ncbi.nlm.nih.gov/38975339/
4. Oprişoreanu, Ana-Maria, Smith, Hannah L, Arya, Sukrat, Talbot, Kevin, Becker, Catherina G. . Interaction of Axonal Chondrolectin with Collagen XIXa1 Is Necessary for Precise Neuromuscular Junction Formation. In Cell reports, 29, 1082-1098.e10. doi:10.1016/j.celrep.2019.09.033. https://pubmed.ncbi.nlm.nih.gov/31665626/
5. Weng, Lin, Hübner, Roland, Claessens, An, Van Marck, Eric, Merregaert, Jozef. . Isolation and characterization of chondrolectin (Chodl), a novel C-type lectin predominantly expressed in muscle cells. In Gene, 308, 21-9. doi:. https://pubmed.ncbi.nlm.nih.gov/12711387/
6. Masuda, Ken, Takano, Atsushi, Oshita, Hideto, Nakamura, Yusuke, Daigo, Yataro. 2011. Chondrolectin is a novel diagnostic biomarker and a therapeutic target for lung cancer. In Clinical cancer research : an official journal of the American Association for Cancer Research, 17, 7712-22. doi:10.1158/1078-0432.CCR-11-0619. https://pubmed.ncbi.nlm.nih.gov/22016508/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen