C57BL/6JCya-Gar1em1/Cya
Common Name
Gar1-KO
Product ID
S-KO-19519
Backgroud
C57BL/6JCya
Strain ID
KOCMP-68147-Gar1-B6J-VC
Status
When using this mouse strain in a publication, please cite “Gar1-KO Mouse (Catalog S-KO-19519) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Gar1-KO
Strain ID
KOCMP-68147-Gar1-B6J-VC
Gene Name
Product ID
S-KO-19519
Gene Alias
Nola1, C430047J18Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 3
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000029643
NCBI RefSeq
NM_026578
Target Region
Exon 3~4
Size of Effective Region
~1.8 kb
Overview of Gene Research
Gar1 is an essential small nucleolar ribonucleoprotein (RNP) protein. It contains two glycine-and arginine-rich GAR domains, localizes in the nucleolus, and is crucial for pre-rRNA processing in yeast, thus being involved in rRNA metabolism [2]. It is also a component of the H/ACA RNP complex, which is responsible for RNA-guided pseudouridylation, an important post-transcriptional RNA modification [4].
In the H/ACA RNP complex, Gar1 has multiple functions. It has a SUMO-interacting motif that mediates the interaction between dyskerin and itself, and this interaction is important for the nuclear and subnuclear localization of dyskerin, which is essential for dyskerin's function as a telomerase-associated protein and an H/ACA ribonucleoprotein [1]. Gar1 also contributes to the RNA-guided and RNA-independent rRNA pseudouridylation activities of the archaeal Cbf5 protein. In vivo and in vitro, it is crucial for the RNA-guide-independent formation of Ψ2607 by Cbf5, and pseudouridylation at an orphan position also relies on the RNA-and Gar1-dependent activity of Cbf5 [5]. Additionally, Gar1 reduces the catalytic barrier in H/ACA RNA-guided pseudouridylation by affecting the activation entropy, helps place the target uridine correctly for the catalytic reaction, and is involved in product release [3]. In archaea, it increases the catalytic activity of Cbf5 in pseudouridylating tRNA by enhancing Cbf5's affinity for tRNA and directly increasing Cbf5's catalytic activity, though it is not involved in product release after tRNA modification [6].
In conclusion, Gar1 is a key protein in the nucleolus, playing essential roles in rRNA processing and RNA pseudouridylation. Its functions in these processes are vital for normal cellular activities. Although no KO/CKO mouse models are directly mentioned in the references, the in-vitro and in-vivo studies on yeast and archaea provide valuable insights into its biological functions, which may potentially be relevant to understanding similar processes in mammals and related disease mechanisms.
References：
1. MacNeil, D E, Lambert-Lanteigne, P, Qin, J, Thibault, P, Autexier, C. 2021. SUMOylation- and GAR1-Dependent Regulation of Dyskerin Nuclear and Subnuclear Localization. In Molecular and cellular biology, 41, . doi:10.1128/MCB.00464-20. https://pubmed.ncbi.nlm.nih.gov/33526451/
2. Girard, J P, Lehtonen, H, Caizergues-Ferrer, M, Tollervey, D, Lapeyre, B. . GAR1 is an essential small nucleolar RNP protein required for pre-rRNA processing in yeast. In The EMBO journal, 11, 673-82. doi:. https://pubmed.ncbi.nlm.nih.gov/1531632/
3. Wang, Peng, Yang, Lijiang, Gao, Yi Qin, Zhao, Xin Sheng. 2015. Accurate placement of substrate RNA by Gar1 in H/ACA RNA-guided pseudouridylation. In Nucleic acids research, 43, 7207-16. doi:10.1093/nar/gkv757. https://pubmed.ncbi.nlm.nih.gov/26206671/
4. De Zoysa, Meemanage D, Yu, Yi-Tao. 2017. Posttranscriptional RNA Pseudouridylation. In The Enzymes, 41, 151-167. doi:10.1016/bs.enz.2017.02.001. https://pubmed.ncbi.nlm.nih.gov/28601221/
5. Fujikane, Ryosuke, Behm-Ansmant, Isabelle, Tillault, Anne-Sophie, Motorin, Yuri, Charpentier, Bruno. 2018. Contribution of protein Gar1 to the RNA-guided and RNA-independent rRNA:Ψ-synthase activities of the archaeal Cbf5 protein. In Scientific reports, 8, 13815. doi:10.1038/s41598-018-32164-0. https://pubmed.ncbi.nlm.nih.gov/30218085/
6. Kamalampeta, Rajashekhar, Kothe, Ute. 2012. Archaeal proteins Nop10 and Gar1 increase the catalytic activity of Cbf5 in pseudouridylating tRNA. In Scientific reports, 2, 663. doi:. https://pubmed.ncbi.nlm.nih.gov/22993689/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
Contact Us
Connect with our experts for your custom animal model needs. Please fill out the form below to start a conversation or request a quote.
Cyagen values your privacy. We’d like to keep you informed about our latest offerings and insights. Your preferences:
You may unsubscribe from these communications at any time. See our Privacy Policy for details on opting out and data protection.
By clicking the button below, you consent to allow Cyagen to store and process the personal information submitted in this form to provide you the content requested.