C57BL/6JCya-Cfap298em1/Cya
Common Name:
Cfap298-KO
Product ID:
S-KO-19574
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Cfap298-KO
Strain ID
KOCMP-68001-Cfap298-B6J-VA
Gene Name
Product ID
S-KO-19574
Gene Alias
1110004E09Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
16
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Cfap298em1/Cya mice (Catalog S-KO-19574) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000023694
NCBI RefSeq
NM_026502
Target Region
Exon 2~3
Size of Effective Region
~2.5 kb
Detailed Document
Overview of Gene Research
Cfap298, also known as C21orf59, is a gene crucial for the transport/assembly of ciliary dyneins [2]. It is conserved in organisms with motile cilia and participates in the cytoplasmic preassembly of various ciliary dyneins, a process essential for cilia motility [2,3]. Motile cilia play critical roles in development and fertility, and thus Cfap298 is of great biological importance [2]. Genetic models, such as zebrafish mutants, are valuable for studying its functions.
In zebrafish, the cfap298tm304 mutant, with defective cilia motility and altered cerebrospinal fluid (CSF) flow, shows a 3D spinal deformity resembling adolescent idiopathic scoliosis (AIS) in humans [1]. This phenotype, more frequent in females, is evolutive during the juvenile phase, has a right convexity, a rotational component, and involves at least one dislocation [1]. Additionally, the reduction of cilia motility in cfap298tm304 mutants slows down CSF transport posteriorly in the central canal and abolishes the spontaneous activity of CSF-contacting neurons [4].
In conclusion, Cfap298 is essential for the cytoplasmic preassembly of ciliary dyneins and cilia motility. The study of cfap298tm304 zebrafish mutants has revealed its role in maintaining spine curvature and has provided insights into the development of AIS-like phenotypes, contributing to our understanding of the disease mechanism [1,4].
References：
1. Marie-Hardy, Laura, Cantaut-Belarif, Yasmine, Pietton, Raphaël, Slimani, Lotfi, Pascal-Moussellard, Hugues. 2021. The orthopedic characterization of cfap298tm304 mutants validate zebrafish to faithfully model human AIS. In Scientific reports, 11, 7392. doi:10.1038/s41598-021-86856-1. https://pubmed.ncbi.nlm.nih.gov/33795825/
2. Yamamoto, Ryosuke, Sahashi, Yui, Shimo-Kon, Rieko, Kurisu, Genji, Kon, Takahide. 2025. Chlamydomonas FBB18 is a ubiquitin-like protein essential for the cytoplasmic preassembly of various ciliary dyneins. In Proceedings of the National Academy of Sciences of the United States of America, 122, e2423948122. doi:10.1073/pnas.2423948122. https://pubmed.ncbi.nlm.nih.gov/40106351/
3. Wang, Limei, Li, Xuecheng, Liu, Guang, Pan, Junmin. 2022. FBB18 participates in preassembly of almost all axonemal dyneins independent of R2TP complex. In PLoS genetics, 18, e1010374. doi:10.1371/journal.pgen.1010374. https://pubmed.ncbi.nlm.nih.gov/36026524/
4. Sternberg, Jenna R, Prendergast, Andrew E, Brosse, Lucie, Delmas, Patrick, Wyart, Claire. 2018. Pkd2l1 is required for mechanoception in cerebrospinal fluid-contacting neurons and maintenance of spine curvature. In Nature communications, 9, 3804. doi:10.1038/s41467-018-06225-x. https://pubmed.ncbi.nlm.nih.gov/30228263/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen