C57BL/6JCya-Rbm25em1/Cya
Common Name:
Rbm25-KO
Product ID:
S-KO-19577
Background:
C57BL/6JCya
Product Type
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Genotype
Sex
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Basic Information
Strain Name
Rbm25-KO
Strain ID
KOCMP-67039-Rbm25-B6J-VA
Gene Name
Product ID
S-KO-19577
Gene Alias
2600011C06Rik; 2610015J01Rik; A130095G20Rik; RNPC7
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
12
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Rbm25em1/Cya mice (Catalog S-KO-19577) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000048155
NCBI RefSeq
NM_027349
Target Region
Exon 3~4
Size of Effective Region
~2.6 kb
Detailed Document
Overview of Gene Research
Rbm25, an RNA-binding protein, is involved in multiple biological processes such as apoptosis, inflammation, and alternative splicing regulation. It is associated with pathways like endoplasmic reticulum stress-related apoptosis and metabolism-associated inflammation pathways. Understanding its function is crucial for revealing the molecular mechanisms of related diseases [1,2].
In an ischemic heart failure (HF) rat model and oxygen-glucose deprivation (OGD) model, RBM25 was significantly upregulated, leading to cardiomyocyte apoptosis by modulating endoplasmic reticulum stress through the CHOP pathway. Inhibiting RBM25 expression improved cardiac function [1].
In RBM25-deficient mice, macrophages showed enhanced pro-inflammatory mediator production, suggesting Rbm25's role in restraining inflammation. Mechanistically, Rbm25 mediates the 14th exon skipping of Acly pre-mRNA, influencing its metabolic activity [2].
In colon cancer, RBM25 ablation suppresses cell growth both in vitro and in vivo by regulating the alternative splicing of MNK2 [3].
In conclusion, Rbm25 plays essential roles in apoptosis, inflammation, and cancer-related processes. Gene knockout or knockdown models in mice and cell-based functional studies have revealed its function in ischemic HF, rheumatoid arthritis, and colon cancer. These findings provide potential therapeutic targets for these diseases.
References:
1. Zhu, Ziwei, Pu, Jie, Li, Yongnan, Zhou, Anyu, Zhang, XiaoWei. 2023. RBM25 regulates hypoxic cardiomyocyte apoptosis through CHOP-associated endoplasmic reticulum stress. In Cell stress & chaperones, 28, 861-876. doi:10.1007/s12192-023-01380-7. https://pubmed.ncbi.nlm.nih.gov/37736860/
2. Zhang, Yunkai, Gao, Ying, Wang, Yujia, Zhan, Zhenzhen, Liu, Xingguang. 2024. RBM25 is required to restrain inflammation via ACLY RNA splicing-dependent metabolism rewiring. In Cellular & molecular immunology, 21, 1231-1250. doi:10.1038/s41423-024-01212-3. https://pubmed.ncbi.nlm.nih.gov/39251781/
3. Zhi, Lili, Chen, Chaoqun, Zhang, Ge, Zhang, Wenjing, Wang, Yang. 2024. RBM25 depletion suppresses the growth of colon cancer cells through regulating alternative splicing of MNK2. In Science China. Life sciences, 67, 2186-2197. doi:10.1007/s11427-023-2582-x. https://pubmed.ncbi.nlm.nih.gov/39110401/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen