C57BL/6JCya-Ccl19em1/Cya
Common Name:
Ccl19-KO
Product ID:
S-KO-19837
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Ccl19-KO
Strain ID
KOCMP-24047-Ccl19-B6J-VA
Gene Name
Product ID
S-KO-19837
Gene Alias
CKb11; ELC; Gm2023; MIP3B; Scya19; exodus-3
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
4
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ccl19em1/Cya mice (Catalog S-KO-19837) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000102957
NCBI RefSeq
NM_011888
Target Region
Exon 2~4
Size of Effective Region
~5.3 kb
Detailed Document
Overview of Gene Research
Ccl19, also known as Chemokine (C-C motif) ligand 19, is a homeostatic chemokine abundantly expressed in the thymus and lymph nodes. It primarily regulates immune cell trafficking and is involved in various biological processes such as T cell activation, immune tolerance, and inflammatory responses. It acts through interaction with its cognate receptor CCR7, and the CCL19-CCR7 axis plays a crucial role in the onset of adaptive immunity, with its signaling pathways influencing viral pathogenesis, immune surveillance, and homeostasis [5].
In cancer research, Ccl19 seems to have a dual-edged role. Ccl19-producing fibroblasts promote the formation of tertiary lymphoid structures (TLSs) in colorectal cancer liver metastasis, enhancing the anti-tumor IgG response. CCL19 treatment also promotes TLS neogenesis and prevents tumor growth in mice [1]. In chimeric antigen receptor (CAR)-T cell therapy for solid tumors, engineering CAR-T cells to secrete CCL19 enhances their expansion, migration, and tumor-suppressing abilities. For example, in hepatocellular carcinoma and pancreatic carcinoma xenografts, 7×19 CAR-T cells (secreting IL-7 and CCL19) show superior tumor suppression compared to conventional CAR-T cells [2,3]. In triple-negative breast cancer, CCL19-expressing dendritic cells are associated with favorable responses to anti-PD-(L)1 immunotherapy, and Ccl19 gene ablation dampens CCR7+CD8+ T cells and tumor elimination in response to anti-PD-1 [4].
In allergic airway disease, Ccl19-deficient mice have less allergic airway inflammation, reduced airway hyper-responsiveness, and less IL-4 and IL-13 production, indicating that CCL19 promotes type 2 T-cell differentiation and allergic airway inflammation [6]. In a mouse model of chronic hepatitis B, CCL19 overexpression leads to rapid clearance of intrahepatic HBV through increased intrahepatic CD8+ T-cell proportion and other immune-related mechanisms [7].
In conclusion, Ccl19 is a key regulator in immune-related processes. Model-based research, especially using gene-knockout mouse models in various disease areas such as cancer, allergic airway disease, and viral infection, has revealed its diverse functions. In cancer, it can either promote anti-tumor immune responses or act as a tumor-supportive factor, while in allergic airway disease it contributes to inflammation, and in HBV infection it aids in virus clearance. These findings provide valuable insights into potential therapeutic strategies targeting Ccl19 in different diseases.
References:
1. Zhang, Yifan, Liu, Guangjian, Zeng, Qianwen, Chen, Zhihang, Kuang, Ming. . CCL19-producing fibroblasts promote tertiary lymphoid structure formation enhancing anti-tumor IgG response in colorectal cancer liver metastasis. In Cancer cell, 42, 1370-1385.e9. doi:10.1016/j.ccell.2024.07.006. https://pubmed.ncbi.nlm.nih.gov/39137726/
2. Pang, Nengzhi, Shi, Jingxuan, Qin, Le, Li, Peng, Zhang, Zhenfeng. 2021. IL-7 and CCL19-secreting CAR-T cell therapy for tumors with positive glypican-3 or mesothelin. In Journal of hematology & oncology, 14, 118. doi:10.1186/s13045-021-01128-9. https://pubmed.ncbi.nlm.nih.gov/34325726/
3. Lu, Li-Li, Xiao, Shu-Xiu, Lin, Zhi-Yuan, Lu, Bin, Wu, Wei-Zhong. 2023. GPC3-IL7-CCL19-CAR-T primes immune microenvironment reconstitution for hepatocellular carcinoma therapy. In Cell biology and toxicology, 39, 3101-3119. doi:10.1007/s10565-023-09821-w. https://pubmed.ncbi.nlm.nih.gov/37853185/
4. Wu, Song-Yang, Zhang, Si-Wei, Ma, Ding, Shao, Zhi-Ming, Jiang, Yi-Zhou. 2023. CCL19+ dendritic cells potentiate clinical benefit of anti-PD-(L)1 immunotherapy in triple-negative breast cancer. In Med (New York, N.Y.), 4, 373-393.e8. doi:10.1016/j.medj.2023.04.008. https://pubmed.ncbi.nlm.nih.gov/37201522/
5. Yan, Yan, Chen, Renfang, Wang, Xu, Lu, Mengji, Hu, Qinxue. 2019. CCL19 and CCR7 Expression, Signaling Pathways, and Adjuvant Functions in Viral Infection and Prevention. In Frontiers in cell and developmental biology, 7, 212. doi:10.3389/fcell.2019.00212. https://pubmed.ncbi.nlm.nih.gov/31632965/
6. Nakano, Keiko, Whitehead, Gregory S, Lyons-Cohen, Miranda R, Cook, Donald N, Nakano, Hideki. 2023. Chemokine CCL19 promotes type 2 T-cell differentiation and allergic airway inflammation. In The Journal of allergy and clinical immunology, 153, 487-502.e9. doi:10.1016/j.jaci.2023.10.024. https://pubmed.ncbi.nlm.nih.gov/37956733/
7. Yan, Yan, Zhao, Wei, Liu, Wei, Xun, Jingna, Davgadorj, Chantsalmaa. 2021. CCL19 enhances CD8+ T-cell responses and accelerates HBV clearance. In Journal of gastroenterology, 56, 769-785. doi:10.1007/s00535-021-01799-8. https://pubmed.ncbi.nlm.nih.gov/34218330/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen