C57BL/6JCya-Scn9aem1/Cya
Common Name:
Scn9a-KO
Product ID:
S-KO-19840
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Scn9a-KO
Strain ID
KOCMP-20274-Scn9a-B6J-VB
Gene Name
Product ID
S-KO-19840
Gene Alias
Nav1.7; PN1; mKIAA4197
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Scn9aem1/Cya mice (Catalog S-KO-19840) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000100064
NCBI RefSeq
NM_001290674
Target Region
Exon 27
Size of Effective Region
~1.0 kb
Detailed Document
Overview of Gene Research
Scn9a encodes the alpha-subunit of the voltage-gated sodium channel, Na(v)1.7, which is strongly expressed in nociceptive neurons and plays a crucial role in peripheral pain sensation [1,3]. It is essential for nociception in humans, and its function is non-redundant in the pain-sensing pathway [1]. Genetic models, such as gene-edited mice, can be used to study its function and the associated pain-related phenotypes.
In three consanguineous families from northern Pakistan, homozygous nonsense mutations in Scn9a (S459X, I767X and W897X) led to a congenital inability to experience pain, as the mutations caused loss of function of Na(v)1.7 [1]. In a Lebanese family, a novel nonsense, homozygous Scn9a pathogenic variant (SCN9A (NM_001365536.1): c.4633G > T, p.(Glu1545*)) was found in three patients with congenital insensitivity to pain, along with urinary incontinence, and two also had osteoporosis and osteoarthritis [2]. In combat athletes and non-athletes, the SCN9A rs6746030 polymorphism may affect pain perception, with the probability of decreased pain tolerance higher in carriers of certain genotypes [3]. The Scn9a R185H mutant mouse model showed pain hypersensitivity and spontaneous pain, indicating the role of this mutation in patients with small fiber neuropathy having chronic pain [4].
In conclusion, Scn9a is essential for nociception. Studies using gene-edited mouse models and human genetic analyses have revealed its key role in pain-related phenotypes. These findings contribute to understanding pain-related diseases, such as congenital insensitivity to pain and small fiber neuropathy, and may help in the development of novel analgesics targeting Na(v)1.7 [1,4].
References:
1. Cox, James J, Reimann, Frank, Nicholas, Adeline K, Gribble, Fiona M, Woods, C Geoffrey. . An SCN9A channelopathy causes congenital inability to experience pain. In Nature, 444, 894-8. doi:. https://pubmed.ncbi.nlm.nih.gov/17167479/
2. Yammine, Tony, Aprahamian, Raffi, Souaid, Mirna, Awwad, Johnny, Farra, Chantal. 2023. Novel SCN9A variant associated with congenital insensitivity to pain. In Molecular biology reports, 50, 6293-6298. doi:10.1007/s11033-023-08507-0. https://pubmed.ncbi.nlm.nih.gov/37231219/
3. Leźnicka, Katarzyna, Pawlak, Maciej, Sawczuk, Marek, Gasiorowska, Agata, Leońska-Duniec, Agata. 2023. SCN9A rs6746030 Polymorphism and Pain Perception in Combat Athletes and Non-Athletes. In Genes, 14, . doi:10.3390/genes14030733. https://pubmed.ncbi.nlm.nih.gov/36981004/
4. Xue, Yaping, Kremer, Mélanie, Muniz Moreno, Maria Del Mar, Herault, Yann, Gaveriaux-Ruff, Claire. 2022. The Human SCN9A R185H Point Mutation Induces Pain Hypersensitivity and Spontaneous Pain in Mice. In Frontiers in molecular neuroscience, 15, 913990. doi:10.3389/fnmol.2022.913990. https://pubmed.ncbi.nlm.nih.gov/35769334/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen