C57BL/6NCya-Tm9sf4em1/Cya
Common Name:
Tm9sf4-KO
Product ID:
S-KO-19848
Background:
C57BL/6NCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Tm9sf4-KO
Strain ID
KOCMP-99237-Tm9sf4-B6N-VA
Gene Name
Product ID
S-KO-19848
Gene Alias
B930079E06; mKIAA0255
Background
C57BL/6NCya
NCBI ID
Modification
Conventional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6NCya-Tm9sf4em1/Cya mice (Catalog S-KO-19848) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000089027
NCBI RefSeq
NM_133847
Target Region
Exon 3~18
Size of Effective Region
~27.5 kb
Detailed Document
Overview of Gene Research
TM9SF4, a member of the transmembrane 9 superfamily, has diverse functions. It is involved in regulating F-actin dynamics, which is crucial for cancer cell motility, and also in processes like autophagy, cell adhesion, and transmembrane domain sorting. It may play roles in multiple signaling pathways such as mTOR-related pathways [1,4].
In ovarian cancer, knockdown of TM9SF4 in cell lines and in vivo (athymic nude mice) reduces cell migration, invasion, tumor growth, and metastasis, suggesting it promotes tumor progression by regulating actin dynamics [1].
In IBD mouse models, genetic knockout of TM9SF4 aggravates disease symptoms, as it promotes inflammation, increases ER stress, disrupts epithelial barrier function, and affects macrophage polarization [2].
In osteoporosis research, TM9SF4 -/- mice show higher bone mass and less lipid accumulation in trabecular bones after ovariectomy, with TM9SF4 -/- bone marrow mesenchymal stem cells having a higher potential to differentiate into osteoblasts [3].
In conclusion, TM9SF4 is essential in processes related to cancer, inflammation, and bone homeostasis. The use of gene knockout mouse models has revealed its role in promoting tumor progression in ovarian cancer, aggravating IBD, and regulating osteoporosis, providing valuable insights into potential therapeutic targets for these diseases.
References：
1. Meng, Zhaoyue, Li, Zhichao, Xie, Mingxu, Jiang, Liwen, Yao, Xiaoqiang. 2022. TM9SF4 is an F-actin disassembly factor that promotes tumor progression and metastasis. In Nature communications, 13, 5728. doi:10.1038/s41467-022-33276-y. https://pubmed.ncbi.nlm.nih.gov/36175399/
2. Xie, Mingxu, Mak, Joyce Wing Yan, Yu, Hongyan, Jiang, Liwen, Yao, Xiaoqiang. 2022. TM9SF4 Is a Crucial Regulator of Inflammation and ER Stress in Inflammatory Bowel Disease. In Cellular and molecular gastroenterology and hepatology, 14, 245-270. doi:10.1016/j.jcmgh.2022.04.002. https://pubmed.ncbi.nlm.nih.gov/35398597/
3. Yu, Libo, Xie, Mingxu, Zhang, Fengjie, Wan, Chao, Yao, Xiaoqiang. 2021. TM9SF4 is a novel regulator in lineage commitment of bone marrow mesenchymal stem cells to either osteoblasts or adipocytes. In Stem cell research & therapy, 12, 573. doi:10.1186/s13287-021-02636-8. https://pubmed.ncbi.nlm.nih.gov/34774100/
4. Sun, Lei, Meng, Zhaoyue, Zhu, Yifei, Jiang, Liwen, Yao, Xiaoqiang. 2017. TM9SF4 is a novel factor promoting autophagic flux under amino acid starvation. In Cell death and differentiation, 25, 368-379. doi:10.1038/cdd.2017.166. https://pubmed.ncbi.nlm.nih.gov/29125601/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen