C57BL/6JCya-Ppp4r2em1/Cya
Common Name:
Ppp4r2-KO
Product ID:
S-KO-19873
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Ppp4r2-KO
Strain ID
KOCMP-232314-Ppp4r2-B6J-VA
Gene Name
Product ID
S-KO-19873
Gene Alias
C230060M08Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
6
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ppp4r2em1/Cya mice (Catalog S-KO-19873) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000063854
NCBI RefSeq
NM_182939
Target Region
Exon 2
Size of Effective Region
~1.6 kb
Detailed Document
Overview of Gene Research
Ppp4r2, a regulatory subunit of the protein phosphatase 4 (PPP4C), is involved in multiple biological processes. It plays a role in DNA damage response (DDR) [2], is associated with the circadian system [3], and may contribute to pathways like Hedgehog signaling [4]. It is also potentially involved in cell differentiation and survival, with implications in neuronal cells and hematopoiesis [1,2].
In neuronal cells, loss of Ppp4r2 function impairs the differentiation of the mouse motor-neuronal cell line NSC-34, an effect counteracted by SMN overexpression. Ppp4r2 may also protect NSC-34 cells from DNA damage-induced apoptosis [1]. In acute myeloid leukemia (AML), genetic inactivation of Ppp4r2 by RNAi in murine hematopoietic stem and progenitor cells and murine myeloid leukemia impairs de-phosphorylation of key DDR proteins, suggesting that the 3p microdeletion affecting Ppp4r2 may contribute to AML pathogenesis [2].
In conclusion, Ppp4r2 is crucial for processes such as neuronal cell differentiation and survival, as well as DNA damage repair in hematopoietic and leukemic cells. The use of gene knockout models in these studies has provided insights into its role in diseases like spinal muscular atrophy-related neuronal cell impairments and AML, highlighting its potential as a therapeutic target in these disease areas.
References:
1. Bosio, Ylenia, Berto, Gaia, Camera, Paola, Claus, Peter, Di Cunto, Ferdinando. 2012. PPP4R2 regulates neuronal cell differentiation and survival, functionally cooperating with SMN. In European journal of cell biology, 91, 662-74. doi:10.1016/j.ejcb.2012.03.002. https://pubmed.ncbi.nlm.nih.gov/22559936/
2. Herzig, Julia K, Bullinger, Lars, Tasdogan, Alpaslan, Döhner, Konstanze, Gaidzik, Verena I. 2017. Protein phosphatase 4 regulatory subunit 2 (PPP4R2) is recurrently deleted in acute myeloid leukemia and required for efficient DNA double strand break repair. In Oncotarget, 8, 95038-95053. doi:10.18632/oncotarget.21119. https://pubmed.ncbi.nlm.nih.gov/29221109/
3. Klemz, Sabrina, Wallach, Thomas, Korge, Sandra, Stanewsky, Ralf, Kramer, Achim. 2021. Protein phosphatase 4 controls circadian clock dynamics by modulating CLOCK/BMAL1 activity. In Genes & development, 35, 1161-1174. doi:10.1101/gad.348622.121. https://pubmed.ncbi.nlm.nih.gov/34301769/
4. Liao, Hengqing, Cai, Jing, Liu, Chen, Cheng, Steven Y, Yue, Shen. 2020. Protein phosphatase 4 promotes Hedgehog signaling through dephosphorylation of Suppressor of fused. In Cell death & disease, 11, 686. doi:10.1038/s41419-020-02843-w. https://pubmed.ncbi.nlm.nih.gov/32826873/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen