C57BL/6JCya-Amotem1/Cya
Common Name:
Amot-KO
Product ID:
S-KO-19878
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Amot-KO
Strain ID
KOCMP-27494-Amot-B6J-VA
Gene Name
Product ID
S-KO-19878
Gene Alias
CAG-2; D0Kist1; Sii6
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
X
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Amotem1/Cya mice (Catalog S-KO-19878) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000112836
NCBI RefSeq
NM_001403387
Target Region
Exon 3~4
Size of Effective Region
~1.4 kb
Detailed Document
Overview of Gene Research
Angiomotin (Amot), a membrane protein, is involved in multiple essential biological functions. It plays a role in regulating cell-cell contact, cellular polarity, and cell growth, and is associated with pathways like the Hippo signaling pathway. Amot's functions are crucial in various biological processes including development, angiogenesis, and neuronal function [2,3,4]. Genetic models, especially KO/CKO mouse models, have been instrumental in studying Amot's functions.
In mice, neuron-specific deletion of Wwc1 and Wwc2, which are related to Amot, leads to reduced Amot protein levels, lower dendritic spine density in the cortex and hippocampus, and impaired cognitive functions. Ectopic expression of Amot partially rescues these neuronal phenotypes, indicating its importance in spinogenesis and cognition [1]. Conditional deletion of Amot and Yap1 in neurons causes a decrease in Purkinje cell dendritic tree complexity, abnormal cerebellar morphology, and motor coordination impairments, suggesting their role in dendrite development [2]. Endothelial-specific deletion of Amot in mice inhibits vascular network migration and expansion, as Amot is required for tip cell migration and filopodia extension, and it functions in relaying forces between fibronectin and the cytoskeleton [4]. In zebrafish and mice, genetic inactivation of Amot cleavage regulators leads to defective angiogenesis, and this phenotype can be rescued by overexpressing a C-terminal Amot cleavage product, showing its role in angiogenesis [5].
In conclusion, Amot is a key regulator in multiple biological processes. Model-based research, especially using KO/CKO mouse models, has revealed its significance in neuronal development, including spinogenesis, dendrite growth, and cognitive function, as well as in vascular development and angiogenesis. Understanding Amot's functions provides insights into the mechanisms of related diseases and may offer potential therapeutic targets.
References:
1. Cao, Runyi, Zhu, Rui, Sha, Zhao, Wang, Yi, Yu, Fa-Xing. 2023. WWC1/2 regulate spinogenesis and cognition in mice by stabilizing AMOT. In Cell death & disease, 14, 491. doi:10.1038/s41419-023-06020-7. https://pubmed.ncbi.nlm.nih.gov/37528078/
2. Rojek, Katarzyna O, Krzemień, Joanna, Doleżyczek, Hubert, Holmgren, Lars, Prószyński, Tomasz J. 2019. Amot and Yap1 regulate neuronal dendritic tree complexity and locomotor coordination in mice. In PLoS biology, 17, e3000253. doi:10.1371/journal.pbio.3000253. https://pubmed.ncbi.nlm.nih.gov/31042703/
3. Wang, Li, Choi, Kyungsuk, Su, Ting, Zheng, Yonggang, Pan, Duojia. 2022. Multiphase coalescence mediates Hippo pathway activation. In Cell, 185, 4376-4393.e18. doi:10.1016/j.cell.2022.09.036. https://pubmed.ncbi.nlm.nih.gov/36318920/
4. Zhang, Yuanyuan, Zhang, Yumeng, Kameishi, Sumako, Cavallo, Federica, Holmgren, Lars. . The Amot/integrin protein complex transmits mechanical forces required for vascular expansion. In Cell reports, 36, 109616. doi:10.1016/j.celrep.2021.109616. https://pubmed.ncbi.nlm.nih.gov/34433061/
5. Wang, Yu, Zhu, Yuwen, Wang, Yebin, Zhang, Ruilin, Yu, Fa-Xing. 2023. Proteolytic activation of angiomotin by DDI2 promotes angiogenesis. In The EMBO journal, 42, e112900. doi:10.15252/embj.2022112900. https://pubmed.ncbi.nlm.nih.gov/37350545/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen