C57BL/6JCya-Cdc14bem1/Cya
Common Name
Cdc14b-KO
Product ID
S-KO-19904
Backgroud
C57BL/6JCya
Strain ID
KOCMP-218294-Cdc14b-B6J-VB
Status
When using this mouse strain in a publication, please cite “Cdc14b-KO Mouse (Catalog S-KO-19904) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Cdc14b-KO
Strain ID
KOCMP-218294-Cdc14b-B6J-VB
Gene Name
Product ID
S-KO-19904
Gene Alias
CDC14B3, Cdc14B1, 2810432N10Rik, A530086E13Rik
Background
C57BL/6JCya
Gene Full Name
CDC14 cell division cycle 14B
Modification
Conventional knockout
NCBI ID
Phenotype
Chromosome
Chr 13 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000039318
NCBI Transcript ID
NM_172587
Target Region
Exon 4~9
Size of Effective Region
~29.3 kb
Overview of Gene Research
Cdc14b, a member of the dual-specificity phosphatases (DUSPs) family, was originally thought to be involved in mitotic exit like its yeast counterpart, but subsequent studies suggest functional specialization in higher eukaryotes. It is now recognized as a key regulator of gene expression and has roles in neuronal development. It may also be involved in meiosis and other developmental processes [1].
In knockout studies, Cdc14b knockout mouse embryonic fibroblasts (MEFs) showed defects in repairing ionizing radiation-induced DNA double-strand breaks (DSBs), especially when Cdc14A levels were low, indicating redundancy between Cdc14B and Cdc14A in DSB repair. Cdc14B deficiency impaired both homologous recombination (HR) and nonhomologous end joining (NHEJ) [4]. In human RPE1 cells, single-and double-knockouts of Cdc14A and Cdc14B did not affect mitotic phases, cytokinesis, or cell proliferation, but cycling Cdc14B KO cells had more frequent and faster-disassembling cilia [3]. In mouse oocytes, overexpression of Cdc14B significantly delayed meiotic resumption, while depletion of Cdc14B led to spontaneous meiotic resumption, suggesting it is a negative regulator of meiotic resumption [2].
In conclusion, Cdc14b has diverse functions in DNA repair, ciliogenesis, and meiotic regulation. The gene knockout models in mice and human cell lines have revealed its role in these biological processes. Understanding Cdc14b is crucial for further exploring normal biological functions and related disease mechanisms, such as potential implications in DNA damage-related diseases and neuronal development disorders.
References：
1. Partscht, Patrick, Schiebel, Elmar. 2023. The diverging role of CDC14B: from mitotic exit in yeast to cell fate control in humans. In The EMBO journal, 42, e114364. doi:10.15252/embj.2023114364. https://pubmed.ncbi.nlm.nih.gov/37493185/
2. Schindler, Karen, Schultz, Richard M. 2009. CDC14B acts through FZR1 (CDH1) to prevent meiotic maturation of mouse oocytes. In Biology of reproduction, 80, 795-803. doi:10.1095/biolreprod.108.074906. https://pubmed.ncbi.nlm.nih.gov/19129509/
3. Partscht, Patrick, Uddin, Borhan, Schiebel, Elmar. 2021. Human cells lacking CDC14A and CDC14B show differences in ciliogenesis but not in mitotic progression. In Journal of cell science, 134, . doi:10.1242/jcs.255950. https://pubmed.ncbi.nlm.nih.gov/33328327/
4. Lin, Han, Ha, Kyungsoo, Lu, Guojun, Zuo, Qiuhong, Zhang, Pumin. 2015. Cdc14A and Cdc14B Redundantly Regulate DNA Double-Strand Break Repair. In Molecular and cellular biology, 35, 3657-68. doi:10.1128/MCB.00233-15. https://pubmed.ncbi.nlm.nih.gov/26283732/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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