C57BL/6JCya-B2mem1/Cya
Common Name:
B2m-KO
Product ID:
S-KO-19919
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
B2m-KO
Strain ID
KOCMP-12010-B2m-B6J-VB
Gene Name
Product ID
S-KO-19919
Gene Alias
Ly-m11; beta2-m; beta2m
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-B2mem1/Cya mice (Catalog S-KO-19919) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000102476
NCBI RefSeq
NM_009735
Target Region
Exon 2~3
Size of Effective Region
~1.9 kb
Detailed Document
Overview of Gene Research
B2m, also known as beta2-microglobulin, is a crucial component of the major histocompatibility complex (MHC) class I molecule. It is essential for presenting tumor antigens to T cells, playing a significant role in tumorigenesis and immune control. Alterations in B2m can impact antigen presentation and are involved in various biological processes related to cancer immunotherapies [1,2,3].
In murine models, knocking out B2m in different cancer cell lines such as MC38, YUMMER2.1, and B16 led to varied responses to anti-PD-1 and IL2 agonist therapies. In MC38 and YUMMER2.1, responses were mediated by CD4+ T cells and natural killer (NK) cells, while in the more aggressive B16, the response was NK-cell-dependent [2]. In human melanoma biopsies, B2M LOH was enriched in non-responders to therapy and was associated with more frequent infiltration of activated NK cells [2].
In conclusion, B2m is vital for MHC-I-mediated antigen presentation and immune response. Gene knockout mouse models have revealed its complex role in cancer immunotherapies, especially in determining the response to immune checkpoint blockade therapies. Understanding B2m's function through these models provides insights into tumor immune escape mechanisms and potential strategies to enhance immunotherapy efficacy [2].
References:
1. Wang, Hanbing, Liu, Baorui, Wei, Jia. 2021. Beta2-microglobulin(B2M) in cancer immunotherapies: Biological function, resistance and remedy. In Cancer letters, 517, 96-104. doi:10.1016/j.canlet.2021.06.008. https://pubmed.ncbi.nlm.nih.gov/34129878/
2. Torrejon, Davis Y, Galvez, Mildred, Abril-Rodriguez, Gabriel, Comin-Anduix, Begoña, Ribas, Antoni. . Antitumor Immune Responses in B2M-Deficient Cancers. In Cancer immunology research, 11, 1642-1655. doi:10.1158/2326-6066.CIR-23-0139. https://pubmed.ncbi.nlm.nih.gov/37801341/
3. Han, Xiaowen, Zhang, Jiayi, Li, Weidong, Gao, Lei, Chen, Hao. 2025. The role of B2M in cancer immunotherapy resistance: function, resistance mechanism, and reversal strategies. In Frontiers in immunology, 16, 1512509. doi:10.3389/fimmu.2025.1512509. https://pubmed.ncbi.nlm.nih.gov/40191187/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen