C57BL/6JCya-Casp1em1/Cya
Common Name:
Casp1-KO
Product ID:
S-KO-19935
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Casp1-KO
Strain ID
KOCMP-12362-Casp1-B6J-VA
Gene Name
Product ID
S-KO-19935
Gene Alias
ICE; Il1bc
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
9
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Casp1em1/Cya mice (Catalog S-KO-19935) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000027015
NCBI RefSeq
NM_009807
Target Region
Exon 4
Size of Effective Region
~0.8 kb
Detailed Document
Overview of Gene Research
Casp1, also known as Caspase-1, is a gene encoding proteins related to cell death. It plays a key role in caspase-1 mediated classical pyroptosis, an important programmed cell death process. Casp1 is associated with various pathways, such as those related to inflammation, immune response, and apoptosis [2,5]. It is involved in the activation of the NLRP3 inflammasome, which is fundamental for interleukin-1β maturation and subsequent inflammatory events [4].
In pancreatic cancer, knockdown of Casp1 inhibited cell viability and migration, and enhanced gemcitabine-induced cell death. Activating Casp1 with a DPP8/DPP9 inhibitor also increased gemcitabine-induced cell death by pyroptosis, suggesting it could be a target for combination therapy [1]. In acute promyelocytic leukemia, ATRA treatment increased and activated Casp1, triggering pyroptosis and differentiation of APL cells, indicating it may act as a suppressor in APL progression [2]. In acute myeloid leukemia, high CASP1 expression was associated with poor prognosis and its inhibition impaired cell proliferation [5]. In atherosclerosis, CASP1 was highly expressed, and the higher its expression, the worse the prognosis [3].
In conclusion, Casp1 is crucial in programmed cell death, especially pyroptosis, and inflammation-related pathways. Gene-knockout studies in various disease models, like pancreatic cancer, leukemia, and atherosclerosis, have revealed its role in disease development, prognosis, and potential as a therapeutic target. These findings contribute to a better understanding of disease mechanisms and the exploration of new treatment strategies.
References:
1. Wang, Xianfeng, Chen, Zheng, Nie, Dingrui, Li, Yangqiu, Zeng, Chengwu. 2023. CASP1 is a target for combination therapy in pancreatic cancer. In European journal of pharmacology, 961, 176175. doi:10.1016/j.ejphar.2023.176175. https://pubmed.ncbi.nlm.nih.gov/37949157/
2. Yu, Xibao, Liu, Xin, Liu, Xuan, Li, Yangqiu, Zeng, Chengwu. 2023. Overexpression of CASP1 triggers acute promyelocytic leukemia cell pyroptosis and differentiation. In European journal of pharmacology, 945, 175614. doi:10.1016/j.ejphar.2023.175614. https://pubmed.ncbi.nlm.nih.gov/36822457/
3. Li, Yongchao, Du, Lihong, Meng, Lingbing, Lv, Chao, Tian, Xinping. . High expression of CASP1 induces atherosclerosis. In Medicine, 103, e37616. doi:10.1097/MD.0000000000037616. https://pubmed.ncbi.nlm.nih.gov/38640260/
4. Heneka, Michael T, Kummer, Markus P, Stutz, Andrea, Latz, Eicke, Golenbock, Douglas T. 2012. NLRP3 is activated in Alzheimer's disease and contributes to pathology in APP/PS1 mice. In Nature, 493, 674-8. doi:10.1038/nature11729. https://pubmed.ncbi.nlm.nih.gov/23254930/
5. Liu, Jing, Zhao, Minyi, Feng, Xiaohui, Zeng, Yunxin, Lin, Dongjun. 2021. Expression and prognosis analyses of CASP1 in acute myeloid leukemia. In Aging, 13, 14088-14108. doi:10.18632/aging.203028. https://pubmed.ncbi.nlm.nih.gov/33999861/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen