C57BL/6JCya-Kdm1aem1/Cya
Common Name:
Kdm1a-KO
Product ID:
S-KO-19993
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Kdm1a-KO
Strain ID
KOCMP-99982-Kdm1a-B6J-VA
Gene Name
Product ID
S-KO-19993
Gene Alias
1810043O07Rik; Aof2; D4Ertd478e; Kdm1; Lsd1; mKIAA0601
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
4
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Kdm1aem1/Cya mice (Catalog S-KO-19993) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000105847
NCBI RefSeq
NM_001347221
Target Region
Exon 5~6
Size of Effective Region
~1.6 kb
Detailed Document
Overview of Gene Research
Kdm1a, also known as Lysine-specific histone demethylase 1A (LSD1), is a histone demethylase that demethylates histone 3 lysine 4 and histone 3 lysine 9 (H3K4me1/2 and H3K9me1/2) using flavin adenine dinucleotide (FAD) as a co-factor. It is involved in multiple biological processes, acting as an epigenetic developmental regulator, and has been implicated in carcinogenesis. It participates in pathways like Wnt/β-catenin, and its dysregulation can lead to abnormal cell growth and differentiation [1,4].
In cancer research, Kdm1a has been found to promote thyroid cancer progression and maintain stemness through the Wnt/β-catenin signaling pathway. Inhibition of Kdm1a using highly selective inhibitors like GSK-LSD1 can significantly inhibit thyroid cancer progression and enhance chemotherapy sensitivity [1]. In acute myeloid leukemia (AML), ORY-1001, a potent and selective KDM1A inhibitor, induces blast differentiation and reduces leukemic stem cell capacity [2]. In gastric cancer patient-derived organoids, genetic and pharmacological inhibition of KDM1A causes growth retardation, and its cancer-supporting functions center on repression of N-myc downstream regulates gene-1 (NDRG1) [3]. In ovarian cancer, knockdown of KDM1A or treatment with its inhibitors sensitizes cancer cells to chemotherapy drugs, and in an orthotopic intrabursal xenograft model, combined treatment significantly reduces tumor growth [5].
In conclusion, Kdm1a is a crucial epigenetic modifier involved in carcinogenesis. Studies using genetic and pharmacological inhibition models, including those similar to KO/CKO mouse models, have revealed its role in promoting cancer progression in various cancer types such as thyroid, AML, gastric, and ovarian cancers. These findings suggest that targeting Kdm1a could be a promising therapeutic strategy for treating these cancers.
References:
1. Zhang, Wei, Ruan, Xianhui, Li, Yaoshuang, Zheng, Xiangqian, Gao, Ming. 2022. KDM1A promotes thyroid cancer progression and maintains stemness through the Wnt/β-catenin signaling pathway. In Theranostics, 12, 1500-1517. doi:10.7150/thno.66142. https://pubmed.ncbi.nlm.nih.gov/35198054/
2. Maes, Tamara, Mascaró, Cristina, Tirapu, Iñigo, Castro-Palomino, Julio Cesar, Buesa, Carlos. 2018. ORY-1001, a Potent and Selective Covalent KDM1A Inhibitor, for the Treatment of Acute Leukemia. In Cancer cell, 33, 495-511.e12. doi:10.1016/j.ccell.2018.02.002. https://pubmed.ncbi.nlm.nih.gov/29502954/
3. Mircetic, Jovan, Camgöz, Aylin, Abohawya, Moustafa, Buchholz, Frank, Stange, Daniel E. 2023. Nuclease technology Screen in Gastric Cancer Patient-Derived Organoids Reveals KDM1A-NDRG1 Axis as a Targetable Vulnerability. In Small methods, 7, e2201605. doi:10.1002/smtd.202201605. https://pubmed.ncbi.nlm.nih.gov/36908010/
4. Ismail, Tayaba, Lee, Hyun-Kyung, Kim, Chowon, Park, Tae Joo, Lee, Hyun-Shik. 2018. KDM1A microenvironment, its oncogenic potential, and therapeutic significance. In Epigenetics & chromatin, 11, 33. doi:10.1186/s13072-018-0203-3. https://pubmed.ncbi.nlm.nih.gov/29921310/
5. Chen, Yihong, Johnson, Jessica D, Jayamohan, Sridharan, Kost, Edward, Sareddy, Gangadhara R. 2024. KDM1A/LSD1 inhibition enhances chemotherapy response in ovarian cancer. In Molecular carcinogenesis, 63, 2026-2039. doi:10.1002/mc.23792. https://pubmed.ncbi.nlm.nih.gov/38990091/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen