C57BL/6JCya-Glg1em1/Cya
Common Name:
Glg1-KO
Product ID:
S-KO-20017
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Glg1-KO
Strain ID
KOCMP-20340-Glg1-B6J-VB
Gene Name
Product ID
S-KO-20017
Gene Alias
CFR; CFR-1; ESL-1; MG-160; MG160; Selel
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
8
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Glg1em1/Cya mice (Catalog S-KO-20017) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000169020
NCBI RefSeq
NM_009149
Target Region
Exon 13~16
Size of Effective Region
~3.7 kb
Detailed Document
Overview of Gene Research
Glg1, also known as Golgi glycoprotein 1, is an intracellular fibroblast growth factor receptor located in the Golgi apparatus [2]. It is associated with various biological processes and diseases.
In Ewing sarcoma, high BCL11B and/or GLG1 immunoreactivity in CD99-positive cases can help diagnose the disease with high specificity and accuracy, potentially serving as specific auxiliary immunohistochemical markers in conjunction with CD99, especially when confirmatory molecular diagnostics are unavailable [1,8]. In cancer, the drug memantine can suppress the growth of malignant glioma and breast cancer cells, upregulate GLG1 mRNA expression, alter its splicing variant profiles, and change its cellular localization, suggesting that the modulation of GLG1 may be one of the mechanisms underlying memantine's cancer-suppressive effects [2]. In gastric cancer, FUT3 promotes cell migration and invasion by synthesizing Lea on ITGA6 and GLG1, with GLG1 affecting vesicle distribution [3].
In yeast, Glg1, the self-glucosylating initiator of glycogen synthesis, was used as an autophagic reporter. Results showed that glycogen autophagy is a non-selective process in nitrogen-starved Komagataella phaffii yeast as both normal Glg1-GFP and mutated Glg1Y212F-GFP were equally well delivered to the vacuole [4]. In the pathogenic yeast Cryptococcus neoformans, Glg1, which initiates glycogen synthesis, was found to influence the formation of both glycogen and the cell wall, as cells missing Glg1 lack α-1,4-glucan in their walls, indicating a link between glycogen and cell wall material [5].
In mice ovarian granulosa cells, Glg1, a downstream target gene of miR-7a2, is down-regulated by taurine, and Glg1 knockdown increases Cyp19a1 expression and estrogen synthesis, suggesting its role in the p38/miR-7a/Glg1/Cyp19a1 signaling pathway regulating estrogen synthesis [6]. A circular RNA derived from GLG1 mRNA (circ_GLG1) promotes colorectal cancer progression by targeting miR-622 and regulating KRAS expression [7].
In conclusion, Glg1 plays diverse and important roles in various biological processes and diseases. In cancer, it is involved in diagnosis, drug-related mechanisms, and tumor progression. In yeast, it helps understand glycogen autophagy. In the ovary, it is part of the estrogen-regulating pathway. The study of Glg1 in different models provides insights into its functions and potential applications in disease diagnosis and treatment.
References:
1. Orth, Martin F, Hölting, Tilman L B, Dallmayer, Marlene, Kirchner, Thomas, Grünewald, Thomas G P. 2020. High Specificity of BCL11B and GLG1 for EWSR1-FLI1 and EWSR1-ERG Positive Ewing Sarcoma. In Cancers, 12, . doi:10.3390/cancers12030644. https://pubmed.ncbi.nlm.nih.gov/32164354/
2. Yamaguchi, Fumio, Hayakawa, Sumio, Kawashima, Shota, Asakura, Takayuki, Oishi, Yumiko. 2022. Antitumor effect of memantine is related to the formation of the splicing isoform of GLG1, a decoy FGF‑binding protein. In International journal of oncology, 61, . doi:10.3892/ijo.2022.5370. https://pubmed.ncbi.nlm.nih.gov/35543162/
3. Wu, Fei, Cao, Li, Zhang, Jinyuan, Qin, Yannan, Huang, Chen. 2024. FUT3 promotes gastric cancer cell migration by synthesizing Lea on ITGA6 and GLG1, affecting adhesion and vesicle distribution. In Life sciences, 359, 123193. doi:10.1016/j.lfs.2024.123193. https://pubmed.ncbi.nlm.nih.gov/39477144/
4. Wijewantha, Nimna V, Kumar, Ravinder, Nazarko, Taras Y. 2024. Glycogen Granules Are Degraded by Non-Selective Autophagy in Nitrogen-Starved Komagataella phaffii. In Cells, 13, . doi:10.3390/cells13060467. https://pubmed.ncbi.nlm.nih.gov/38534311/
5. Loza, Liza, Doering, Tamara L. 2024. A fungal protein organizes both glycogen and cell wall glucans. In Proceedings of the National Academy of Sciences of the United States of America, 121, e2319707121. doi:10.1073/pnas.2319707121. https://pubmed.ncbi.nlm.nih.gov/38743622/
6. Li, Liuhui, Lu, Chenyang, Zhang, Di, Liu, Hui, Cui, Sheng. 2022. Taurine promotes estrogen synthesis by regulating microRNA-7a2 in mice ovarian granulosa cells. In Biochemical and biophysical research communications, 626, 129-134. doi:10.1016/j.bbrc.2022.07.084. https://pubmed.ncbi.nlm.nih.gov/35988296/
7. Hao, Shuhong, Qu, Rongfeng, Hu, Chunmei, Wang, Min, Li, Yarong. 2020. A Circular RNA Derived from Golgi Glycoprotein 1 mRNA Regulates KRAS Expression and Promotes Colorectal Cancer Progression by Targeting microRNA-622. In OncoTargets and therapy, 13, 12637-12648. doi:10.2147/OTT.S284032. https://pubmed.ncbi.nlm.nih.gov/33335404/
8. Baldauf, Michaela C, Orth, Martin F, Dallmayer, Marlene, Kirchner, Thomas, Grünewald, Thomas G P. 2017. Robust diagnosis of Ewing sarcoma by immunohistochemical detection of super-enhancer-driven EWSR1-ETS targets. In Oncotarget, 9, 1587-1601. doi:10.18632/oncotarget.20098. https://pubmed.ncbi.nlm.nih.gov/29416716/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen