C57BL/6JCya-Ddb2em1/Cya
Common Name
Ddb2-KO
Product ID
S-KO-20022
Backgroud
C57BL/6JCya
Strain ID
KOCMP-107986-Ddb2-B6J-VB
Status
When using this mouse strain in a publication, please cite “Ddb2-KO Mouse (Catalog S-KO-20022) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
The standard delivery applies for a guaranteed minimum of three heterozygous carriers. Breeding services for homozygous carriers and/or specified sex are available.
Basic Information
Strain Name
Ddb2-KO
Strain ID
KOCMP-107986-Ddb2-B6J-VB
Gene Name
Product ID
S-KO-20022
Gene Alias
2610043A19Rik
Background
C57BL/6JCya
Gene Full Name
damage specific DNA binding protein 2
Modification
Conventional knockout
NCBI ID
Phenotype
Chromosome
Chr 2 (Mouse)
Application
--
Datasheet
Strain Description
Ensembl Transcript ID
ENSMUST00000028696
NCBI Transcript ID
NM_028119
Target Region
Exon 5~7
Size of Effective Region
~1.7 kb
Overview of Gene Research
Ddb2, also known as damage-specific DNA-binding protein 2, is initially identified as a DNA damage recognition factor crucial for global genomic nucleotide excision repair (GG-NER) in human cells. It is also involved in multiple essential biological processes such as chromatin remodeling, gene transcription, cell cycle regulation, and protein decay [1].
In cancer research, Ddb2 shows diverse roles. In pancreatic ductal adenocarcinoma (PDAC), its reduced expression in cancer tissues correlates with shorter disease-free survival. Overexpression of Ddb2 in PDAC cells represses epithelial-to-mesenchymal transition (EMT), reduces cell migration and invasion, and sensitizes cells to chemotherapy drugs like 5-fluorouracil, oxaliplatin, and gemcitabine, suggesting its tumor-suppressive effects [2]. In breast and ovarian teratoma tissues, high Ddb2 levels are associated with lower CDT2 levels, and Ddb2 regulates DNA replication by degrading CDT2 [3]. In lung adenocarcinoma (LUAD), MBD2 binds to the methylated CpG DNA within the DDB2 promoter to repress DDB2 expression, promoting tumor metastasis [4]. In hepatocellular carcinoma cells, TRABID deubiquitinates DDB2 to promote cell proliferation [5].
In conclusion, Ddb2 plays essential roles in DNA repair, cell cycle regulation, and protein degradation. Its study in cancer models reveals its potential as a prognostic biomarker and therapeutic target in various cancers, especially in PDAC, where it may help predict chemotherapy response and prognosis. Understanding Ddb2's functions contributes to a deeper comprehension of cancer development mechanisms and may guide the development of new cancer therapies.
References：
1. Gilson, Pauline, Drouot, Guillaume, Witz, Andréa, Becuwe, Philippe, Harlé, Alexandre. 2019. Emerging Roles of DDB2 in Cancer. In International journal of molecular sciences, 20, . doi:10.3390/ijms20205168. https://pubmed.ncbi.nlm.nih.gov/31635251/
2. Dardare, Julie, Witz, Andréa, Betz, Margaux, Harlé, Alexandre, Gilson, Pauline. 2022. DDB2 represses epithelial-to-mesenchymal transition and sensitizes pancreatic ductal adenocarcinoma cells to chemotherapy. In Frontiers in oncology, 12, 1052163. doi:10.3389/fonc.2022.1052163. https://pubmed.ncbi.nlm.nih.gov/36568213/
3. Wu, Xiaojun, Yu, Min, Zhang, Zhuxia, Xie, Ni, Lu, Fei. 2021. DDB2 regulates DNA replication through PCNA-independent degradation of CDT2. In Cell & bioscience, 11, 34. doi:10.1186/s13578-021-00540-5. https://pubmed.ncbi.nlm.nih.gov/33557942/
4. Zhang, Lei, Wang, Siyuan, Wu, Guo-Rao, Yong, Tuying, Wang, Cong-Yi. 2023. MBD2 facilitates tumor metastasis by mitigating DDB2 expression. In Cell death & disease, 14, 303. doi:10.1038/s41419-023-05804-1. https://pubmed.ncbi.nlm.nih.gov/37142578/
5. Chen, Yaping, Zhang, Xiaofei. 2022. TRABID targets DDB2 for deubiquitination to promote proliferation of hepatocellular carcinoma cells. In Biochemical and biophysical research communications, 625, 23-30. doi:10.1016/j.bbrc.2022.07.109. https://pubmed.ncbi.nlm.nih.gov/35944360/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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