C57BL/6JCya-Ddx52em1/Cya
Common Name:
Ddx52-KO
Product ID:
S-KO-20036
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Ddx52-KO
Strain ID
KOCMP-78394-Ddx52-B6J-VA
Gene Name
Product ID
S-KO-20036
Gene Alias
2700029C06Rik; ROK1
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
11
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ddx52em1/Cya mice (Catalog S-KO-20036) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000049257
NCBI RefSeq
NM_030096
Target Region
Exon 3~4
Size of Effective Region
~3.1 kb
Detailed Document
Overview of Gene Research
Ddx52, also known as ROK1, is an ATP-dependent RNA helicase. It is involved in multiple biological processes. In zebrafish, it functions as a growth switch during juvenile stages, maintaining the level of 47S precursor ribosomal RNA [3].
In the context of human diseases, it has been implicated in cancer development.
In prostate cancer, Ddx52 expression is upregulated, especially in advanced stages. Knockdown of Ddx52 inhibits prostate cancer cell growth both in vitro and in vivo by regulating c-Myc signaling [2]. Similarly, in melanoma, Ddx52 is overexpressed in tumor tissues compared to non-tumor tissues. Downregulation of Ddx52 suppresses melanoma cell proliferation in vitro and in nude mouse xenografts by targeting c-Myc [4]. In lung adenocarcinoma (LUAD), Ddx52 expression is higher in tumor tissues, serving as a negative prognostic marker, and influencing LUAD progression via multiple signaling pathways [1].
In conclusion, Ddx52 is crucial for growth regulation in zebrafish and has a significant impact on cancer development, especially in prostate cancer, melanoma, and LUAD. Studies using knockdown models in these cancer types have revealed its role in promoting cell growth and progression, suggesting it could be a potential therapeutic target in these disease areas.
References：
1. Xu, Mingming, Yang, Mingjun. 2023. DDX52 gene expression in LUAD tissues indicates potential as a prognostic biomarker and therapeutic target. In Scientific reports, 13, 17434. doi:10.1038/s41598-023-44347-5. https://pubmed.ncbi.nlm.nih.gov/37833424/
2. Yu, Wandong, Ma, Hangbin, Li, Junhong, Zhang, Jun, Shi, Guowei. 2021. DDX52 knockdown inhibits the growth of prostate cancer cells by regulating c-Myc signaling. In Cancer cell international, 21, 430. doi:10.1186/s12935-021-02128-y. https://pubmed.ncbi.nlm.nih.gov/34399732/
3. Tseng, Tzu-Lun, Wang, Ying-Ting, Tsao, Chang-Yu, Poss, Kenneth D, Chen, Chen-Hui. 2021. The RNA helicase Ddx52 functions as a growth switch in juvenile zebrafish. In Development (Cambridge, England), 148, . doi:10.1242/dev.199578. https://pubmed.ncbi.nlm.nih.gov/34323273/
4. Wang, Qiang, Qian, Leqi, Tao, Mengyuan, Liu, Jiaqi, Qi, Fa-Zhi. . Knockdown of DEAD-box RNA helicase 52 (DDX52) suppresses the proliferation of melanoma cells in vitro and of nude mouse xenografts by targeting c-Myc. In Bioengineered, 12, 3539-3549. doi:10.1080/21655979.2021.1950283. https://pubmed.ncbi.nlm.nih.gov/34233596/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen