C57BL/6JCya-Apmapem1/Cya
Common Name:
Apmap-KO
Product ID:
S-KO-20185
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Apmap-KO
Strain ID
KOCMP-71881-Apmap-B6J-VB
Gene Name
Product ID
S-KO-20185
Gene Alias
2310001A20Rik
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Apmapem1/Cya mice (Catalog S-KO-20185) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000046399
NCBI RefSeq
NM_027977
Target Region
Exon 3
Size of Effective Region
~1.8 kb
Detailed Document
Overview of Gene Research
Apmap, also known as adipocyte plasma membrane-associated protein, is a glycosyl type II transmembrane protein mainly distributed in the plasma membrane and endoplasmic reticulum of adipocytes. It is an arylesterase that plays a crucial role in adipogenesis. Apmap is involved in multiple biological processes such as lipid transport, epithelial-mesenchymal transition, and may be associated with pathways like Wnt/β-catenin. It also has implications in maintaining endoplasmic reticulum-associated lipid and lipoprotein homeostasis [2,3,6].
In cancer cells, loss-of-function studies using CRISPR knockout screens identified Apmap as a regulator of susceptibility to antibody-dependent cellular phagocytosis (ADCP). Loss of Apmap synergizes with tumour antigen-targeting monoclonal antibodies and/or CD47-blocking monoclonal antibodies to drive increased phagocytosis across various cancer cell types and inhibit tumour growth in mice [1]. In cervical cancer, knockdown of Apmap significantly inhibited the migration ability of cancer cells, indicating its role in promoting epithelial-mesenchymal transition and metastasis through activating the Wnt/β-catenin pathway [3]. In a mouse model of Alzheimer's disease, constitutive deletion of Apmap worsened spatial memory phenotype and led to increased Aβ production and deposition into senile plaques [4]. In Rex rabbits, knockdown of FTO, which can increase Apmap expression, affected intramuscular fat deposition as Apmap promotes adipocyte differentiation [5]. Knockout of full-length Apmap in mice led to an improved metabolic phenotype upon diet-induced obesity, with enhanced insulin sensitivity, preserved glucose tolerance, etc., and Apmap was found to interact with extracellular collagen cross-linking matrix proteins [7].
In conclusion, Apmap has diverse functions in adipogenesis, lipid and lipoprotein homeostasis, and is involved in various disease conditions such as cancer, Alzheimer's disease, and metabolic disorders. Gene knockout mouse models have been instrumental in revealing these functions, providing valuable insights into the underlying mechanisms and potential therapeutic targets for these diseases.
References:
1. Kamber, Roarke A, Nishiga, Yoko, Morton, Bhek, Sage, Julien, Bassik, Michael C. 2021. Inter-cellular CRISPR screens reveal regulators of cancer cell phagocytosis. In Nature, 597, 549-554. doi:10.1038/s41586-021-03879-4. https://pubmed.ncbi.nlm.nih.gov/34497417/
2. Luo, Gang, Mu, Jinzhan, Wang, Shuhui, Dong, Xianggui, Ren, Zhanjun. 2022. Association of blood APMAP content and meat quality trait in Rex rabbits. In Animal biotechnology, 34, 974-979. doi:10.1080/10495398.2021.2007117. https://pubmed.ncbi.nlm.nih.gov/35001846/
3. Zhu, Xiuting, Xiang, Zijin, Zou, Lingxiao, Peng, Xiangdong, Xu, Dabao. 2021. APMAP Promotes Epithelial-Mesenchymal Transition and Metastasis of Cervical Cancer Cells by Activating the Wnt/β-catenin Pathway. In Journal of Cancer, 12, 6265-6273. doi:10.7150/jca.59595. https://pubmed.ncbi.nlm.nih.gov/34539899/
4. Gerber, Hermeto, Mosser, Sebastien, Boury-Jamot, Benjamin, Magara, Fulvio, Fraering, Patrick C. 2019. The APMAP interactome reveals new modulators of APP processing and beta-amyloid production that are altered in Alzheimer's disease. In Acta neuropathologica communications, 7, 13. doi:10.1186/s40478-019-0660-3. https://pubmed.ncbi.nlm.nih.gov/30704515/
5. Luo, Gang, Hong, Tingting, Yu, Lin, Ren, Zhanjun. 2023. FTO Regulated Intramuscular Fat by Targeting APMAP Gene via an m6A-YTHDF2-dependent Manner in Rex Rabbits. In Cells, 12, . doi:10.3390/cells12030369. https://pubmed.ncbi.nlm.nih.gov/36766716/
6. Paul, Blessy, Merta, Holly, Ugrankar-Banerjee, Rupali, Farber, Steven A, Henne, W Mike. 2024. Paraoxonase-like APMAP maintains endoplasmic reticulum-associated lipid and lipoprotein homeostasis. In bioRxiv : the preprint server for biology, , . doi:10.1101/2024.01.26.577049. https://pubmed.ncbi.nlm.nih.gov/38328083/
7. Pessentheiner, Ariane R, Huber, Katharina, Pelzmann, Helmut J, Bilban, Martin, Bogner-Strauss, Juliane G. 2017. APMAP interacts with lysyl oxidase-like proteins, and disruption of Apmap leads to beneficial visceral adipose tissue expansion. In FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 31, 4088-4103. doi:10.1096/fj.201601337R. https://pubmed.ncbi.nlm.nih.gov/28559441/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen