C57BL/6JCya-Kyat1em1/Cya
Common Name:
Kyat1-KO
Product ID:
S-KO-20211
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Kyat1-KO
Strain ID
KOCMP-70266-Kyat1-B6J-VB
Gene Name
Product ID
S-KO-20211
Gene Alias
2010009K05Rik; Ccbl1; Gtk; Kat1; KatI
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Kyat1em1/Cya mice (Catalog S-KO-20211) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000113663
NCBI RefSeq
NM_172404
Target Region
Exon 5~14
Size of Effective Region
~3.2 kb
Detailed Document
Overview of Gene Research
KYAT1, also known as kynurenine aminotransferase 1 or CCBL1, is a bi-functional enzyme that plays a major role in Se-methylselenocysteine (MSC) metabolism. It can catalyze transamination and beta-elimination activities with MSC as a substrate, producing methylselenol (CH3SeH), a cytotoxic selenium metabolite causing apoptosis in cancer cells [3]. It is also involved in the kynurenine pathway, which is associated with the production of neuroactive metabolites like kynurenic acid [5].
In the context of disease, KYAT1 has been implicated in several conditions. In Prader-Willi syndrome, its differential expression was observed in liver steatosis, with levels increasing from grade 1 to grade 3, suggesting its potential as a biomarker for detecting this condition [1]. In rats exposed to a heavy-metal mixture, altered levels of KYAT1 were associated with neurological impairments, hypothesizing a link between the dysregulation of ferroptosis and kynurenine pathways and neurological damage [2]. In critically ill patients, KYAT1 mRNA expression in skeletal muscle was reduced, and this lower expression was associated with a lower risk of weakness. Therapeutic interventions like withholding parenteral nutrition in the first ICU week led to higher KYAT1 expression [4]. In COVID-19 patients with ARDS, KYAT1 was among the proteins with high variable importance in a random forest model for COVID-19 [7]. In pink-eyed white mink, KYAT1 was suggested as a possible gene related to body weight [6].
In conclusion, KYAT1 is an enzyme with key functions in metabolism and is associated with multiple disease-related processes. Its involvement in liver steatosis in Prader-Willi syndrome, neurological impairments in heavy-metal-exposed rats, critical illness in humans, and body weight in mink highlights its significance in understanding these biological and disease conditions. The study of KYAT1 in these contexts provides insights into potential biomarker discovery and disease mechanisms [1,2,4,6,7].
References:
1. Pascut, Devis, Giraudi, Pablo J, Banfi, Cristina, Grugni, Graziano, Sartorio, Alessandro. 2023. Proteome profiling identifies circulating biomarkers associated with hepatic steatosis in subjects with Prader-Willi syndrome. In Frontiers in endocrinology, 14, 1254778. doi:10.3389/fendo.2023.1254778. https://pubmed.ncbi.nlm.nih.gov/38034016/
2. Xie, Jie, Zhou, Fankun, Ouyang, Lu, Feng, Chang, Fan, Guangqin. 2023. Insight into the effect of a heavy metal mixture on neurological damage in rats through combined serum metabolomic and brain proteomic analyses. In The Science of the total environment, 895, 165009. doi:10.1016/j.scitotenv.2023.165009. https://pubmed.ncbi.nlm.nih.gov/37353033/
3. Selvam, Arun Kumar, Björnstedt, Mikael. 2020. A Novel Assay Method to Determine the β-Elimination of Se-Methylselenocysteine to Monomethylselenol by Kynurenine Aminotransferase 1. In Antioxidants (Basel, Switzerland), 9, . doi:10.3390/antiox9020139. https://pubmed.ncbi.nlm.nih.gov/32033380/
4. Vanhorebeek, Ilse, Gunst, Jan, Casaer, Michaël P, Gosselink, Rik, Van den Berghe, Greet. 2023. Skeletal Muscle Myokine Expression in Critical Illness, Association With Outcome and Impact of Therapeutic Interventions. In Journal of the Endocrine Society, 7, bvad001. doi:10.1210/jendso/bvad001. https://pubmed.ncbi.nlm.nih.gov/36726836/
5. Brown, Samara J, Brown, Amelia M, Purves-Tyson, Tertia D, Shannon Weickert, Cynthia, Newell, Kelly A. 2021. Alterations in the kynurenine pathway and excitatory amino acid transporter-2 in depression with and without psychosis: Evidence of a potential astrocyte pathology. In Journal of psychiatric research, 147, 203-211. doi:10.1016/j.jpsychires.2021.12.039. https://pubmed.ncbi.nlm.nih.gov/35063739/
6. Shi, Hongyu, Liu, Linling, Larsen, Peter Foged, Zhang, Haihua, Liu, Zongyue. 2024. Genomic Regions Associated with Growth and Reproduction Traits in Pink-Eyed White Mink. In Genes, 15, . doi:10.3390/genes15091142. https://pubmed.ncbi.nlm.nih.gov/39336733/
7. Molinero, Marta, Gómez, Silvia, Benítez, Iván D, Barbé, Ferran, de Gonzalo-Calvo, David. 2022. Multiplex protein profiling of bronchial aspirates reveals disease-, mortality- and respiratory sequelae-associated signatures in critically ill patients with ARDS secondary to SARS-CoV-2 infection. In Frontiers in immunology, 13, 942443. doi:10.3389/fimmu.2022.942443. https://pubmed.ncbi.nlm.nih.gov/35967328/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen