C57BL/6JCya-Rad54lem1/Cya
Common Name:
Rad54l-KO
Product ID:
S-KO-20258
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Rad54l-KO
Strain ID
KOCMP-19366-Rad54l-B6J-VB
Gene Name
Product ID
S-KO-20258
Gene Alias
RAD54
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
4
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Rad54lem1/Cya mice (Catalog S-KO-20258) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000102704
NCBI RefSeq
NM_009015
Target Region
Exon 4
Size of Effective Region
~1.5 kb
Detailed Document
Overview of Gene Research
Rad54l, a DNA motor protein, is a key member of the SWI2/SNF2 protein family. It plays multiple roles in homologous recombination DNA repair (HR), such as catalyzing the reversal and restoration of model replication forks in vitro. It is involved in regulating DNA replication dynamics, and the homologous recombination repair pathway is crucial for maintaining genome stability [2,3,4,5,6,7].
In cancer research, high Rad54l expression in bladder cancer tissue is associated with poor prognosis, promoting abnormal cell proliferation by regulating the cell cycle and senescence, and may be associated with p53, p21, and pRB [1]. In hepatocellular carcinoma, its up-regulation is positively correlated with tumor pathological staging and negatively associated with patient overall survival, and it is correlated with Edmondson-Steiner grade and the proliferation-related gene Ki67 [3]. In early-stage lung adenocarcinoma, high expression of Rad54l is related to worse overall survival, and it is an independent prognostic factor [6]. Also, in pan-cancer analysis, Rad54l is highly expressed in most tumors, associated with survival outcomes, immune cell infiltration, TMB, MSI, and immune checkpoints, and promotes the proliferation and migration of HCC cells in vitro [7].
In conclusion, Rad54l is essential for homologous recombination DNA repair and regulating DNA replication. Its dysregulation is closely associated with the progression of various cancers including bladder, liver, and lung adenocarcinoma. Understanding Rad54l's function through research can provide potential prognostic biomarkers and treatment targets for these cancers.
References：
1. Wang, Yinzhao, Zhou, Tailai, Chen, Hengxing, Dao, Pinghong, Chen, Minfeng. 2022. Rad54L promotes bladder cancer progression by regulating cell cycle and cell senescence. In Medical oncology (Northwood, London, England), 39, 185. doi:10.1007/s12032-022-01751-7. https://pubmed.ncbi.nlm.nih.gov/36071250/
2. Uhrig, Mollie E, Sharma, Neelam, Maxwell, Petey, Mazin, Alexander V, Wiese, Claudia. . Disparate requirements for RAD54L in replication fork reversal. In Nucleic acids research, 52, 12390-12404. doi:10.1093/nar/gkae828. https://pubmed.ncbi.nlm.nih.gov/39315725/
3. Li, Hongda, Zhuang, Haiwen, Gu, Tengfei, Xu, Sanrong, Zhou, Qing. 2023. RAD54L promotes progression of hepatocellular carcinoma via the homologous recombination repair pathway. In Functional & integrative genomics, 23, 128. doi:10.1007/s10142-023-01060-w. https://pubmed.ncbi.nlm.nih.gov/37071224/
4. Uhrig, Mollie E, Sharma, Neelam, Maxwell, Petey, Mazin, Alexander V, Wiese, Claudia. 2024. Disparate requirements for RAD54L in replication fork reversal. In bioRxiv : the preprint server for biology, , . doi:10.1101/2023.07.26.550704. https://pubmed.ncbi.nlm.nih.gov/37546955/
5. Selemenakis, Platon, Sharma, Neelam, Uhrig, Mollie E, Sung, Patrick, Wiese, Claudia. 2022. RAD51AP1 and RAD54L Can Underpin Two Distinct RAD51-Dependent Routes of DNA Damage Repair via Homologous Recombination. In Frontiers in cell and developmental biology, 10, 866601. doi:10.3389/fcell.2022.866601. https://pubmed.ncbi.nlm.nih.gov/35652094/
6. Zheng, Shaopeng, Yao, Lintong, Li, Fasheng, Lanuti, Michael, Zhou, Haiyu. 2021. Homologous recombination repair rathway and RAD54L in early-stage lung adenocarcinoma. In PeerJ, 9, e10680. doi:10.7717/peerj.10680. https://pubmed.ncbi.nlm.nih.gov/33628633/
7. Zhou, Yongzhen, Qiu, Chenjie, Fu, Qingsheng, Qin, Xihu, Wu, Baoqiang. 2023. Pan-Cancer Analysis of Oncogenic Role of RAD54L and Experimental Validation in Hepatocellular Carcinoma. In Journal of inflammation research, 16, 3997-4017. doi:10.2147/JIR.S426558. https://pubmed.ncbi.nlm.nih.gov/37719938/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen