C57BL/6JCya-Zcwpw1em1/Cya
Common Name:
Zcwpw1-KO
Product ID:
S-KO-20289
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
Contact for Pricing
Basic Information
Strain Name
Zcwpw1-KO
Strain ID
KOCMP-381678-Zcwpw1-B6J-VA
Gene Name
Product ID
S-KO-20289
Gene Alias
Gm1053
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
5
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Zcwpw1em1/Cya mice (Catalog S-KO-20289) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000035852
NCBI RefSeq
NM_001005426
Target Region
Exon 3
Size of Effective Region
~1.6 kb
Detailed Document
Overview of Gene Research
Zcwpw1, without common aliases mentioned in the references, is an essential gene involved in meiotic processes. It contains H3K4me3 and H3K36me3 recognition domains and is associated with the PRDM9-mediated pathway in meiosis. During meiosis, it plays a crucial role in homologous recombination, which is vital for generating genetic diversity and the proper formation of germ cells [1,2,3,5,6].
Male Zcwpw1 knockout mice show severe DSB repair and synapsis defects, with persistent DMC1 foci, ultimately leading to sterility. This indicates that Zcwpw1 is essential for synapsis and fertility in male mice [1]. In male mice, loss of Zcwpw1 causes complete failure of synapsis, meiotic arrest at the zygotene to pachytene stage, incomplete DNA double-strand break repair, and lack of crossover formation, resulting in male infertility. In contrast, in female mice, deletion of Zcwpw1 only slows down meiosis prophase I progression, and Zcwpw1-/-female mice have normal fertility until mid-adulthood [3]. A homozygous missense mutation in ZCWPW1 in humans also causes male infertility with sperm head defects and high DNA fragmentation [7]. Additionally, Zcwpw1 has been associated with Alzheimer's disease risk, as rare-variant burden in ZCWPW1 highlighted it as a potential driver of an AD-genome-wide association study locus [4].
In conclusion, Zcwpw1 is indispensable for meiosis synapsis in males, playing a key role in the repair of double-strand breaks and synapsis during meiosis. The gene knockout mouse models have clearly demonstrated its importance in male fertility. Its association with Alzheimer's disease also indicates its potential significance in neurodegenerative disease research, providing valuable insights into both reproductive biology and neurodegenerative disease mechanisms.
References:
1. Wells, Daniel, Bitoun, Emmanuelle, Moralli, Daniela, Green, Catherine, Myers, Simon R. 2020. ZCWPW1 is recruited to recombination hotspots by PRDM9 and is essential for meiotic double strand break repair. In eLife, 9, . doi:10.7554/eLife.53392. https://pubmed.ncbi.nlm.nih.gov/32744506/
2. Huang, Tao, Yuan, Shenli, Gao, Lei, Chen, Zi-Jiang, Liu, Hongbin. 2020. The histone modification reader ZCWPW1 links histone methylation to PRDM9-induced double-strand break repair. In eLife, 9, . doi:10.7554/eLife.53459. https://pubmed.ncbi.nlm.nih.gov/32374261/
3. Li, Miao, Huang, Tao, Li, Meng-Jing, Liu, Hong-Bin, Liu, Kui. 2019. The histone modification reader ZCWPW1 is required for meiosis prophase I in male but not in female mice. In Science advances, 5, eaax1101. doi:10.1126/sciadv.aax1101. https://pubmed.ncbi.nlm.nih.gov/31453335/
4. Holstege, Henne, Hulsman, Marc, Charbonnier, Camille, Bellenguez, Céline, Lambert, Jean-Charles. 2022. Exome sequencing identifies rare damaging variants in ATP8B4 and ABCA1 as risk factors for Alzheimer's disease. In Nature genetics, 54, 1786-1794. doi:10.1038/s41588-022-01208-7. https://pubmed.ncbi.nlm.nih.gov/36411364/
5. Yuan, Shenli, Huang, Tao, Bao, Ziyou, Liu, Hongbin, Chen, Zi-Jiang. 2022. The histone modification reader ZCWPW1 promotes double-strand break repair by regulating cross-talk of histone modifications and chromatin accessibility at meiotic hotspots. In Genome biology, 23, 187. doi:10.1186/s13059-022-02758-z. https://pubmed.ncbi.nlm.nih.gov/36068616/
6. Mahgoub, Mohamed, Paiano, Jacob, Bruno, Melania, Nussenzweig, André, Macfarlan, Todd S. 2020. Dual histone methyl reader ZCWPW1 facilitates repair of meiotic double strand breaks in male mice. In eLife, 9, . doi:10.7554/eLife.53360. https://pubmed.ncbi.nlm.nih.gov/32352380/
7. Song, Yuelin, Guo, Juncen, Zhou, Yanling, Zhang, Guohui, Wang, Hongjing. 2024. A loss-of-function variant in ZCWPW1 causes human male infertility with sperm head defect and high DNA fragmentation. In Reproductive health, 21, 18. doi:10.1186/s12978-024-01746-9. https://pubmed.ncbi.nlm.nih.gov/38310235/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen