C57BL/6JCya-Rab7em1/Cya
Common Name:
Rab7-KO
Product ID:
S-KO-20314
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Rab7-KO
Strain ID
KOCMP-19349-Rab7-B6J-VB
Gene Name
Product ID
S-KO-20314
Gene Alias
Rab7a
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
6
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Rab7em1/Cya mice (Catalog S-KO-20314) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000113600
NCBI RefSeq
NM_009005
Target Region
Exon 3
Size of Effective Region
~1.4 kb
Detailed Document
Overview of Gene Research
Rab7, a member of the RAS oncogene family, is a small GTPase that plays a crucial role in regulating endomembrane trafficking pathways, especially those related to endocytic and autophagic processes [4]. It cycles between an active GTP-bound state and an inactive GDP-bound state, interacting with various effector proteins to control multiple cellular functions [1,6].
Endothelial haploinsufficiency of Rab7 in mice led to spontaneous pulmonary hypertension (PH), as Rab7-silenced endothelial cells showed impaired angiogenesis, senescent cell expansion, and disrupted endolysosomal trafficking, suggesting a key role in maintaining endothelial function and lung vascular homeostasis [2]. In oocytes, the activity of Rab7 is essential for the regulation of mitophagy during meiosis and oocyte quality control in ovarian aging. When its activity was decreased in CCCP-treated oocytes, defects in meiosis and mitophagy occurred, which could be rescued by active Rab7 or its activator [3]. In diabetic cardiomyopathy, Rab7-Rilp-mediated microlipophagy may be a potential target for treating lipid toxicity, as treating DbCM mice with the Rab7 activator ML-098 enhanced Rilp level and rescued cardiac dysfunction [5].
In conclusion, Rab7 is a vital regulator in multiple biological processes, including endomembrane trafficking, mitophagy, and endothelial function. Gene-knockout models, such as Rab7-silenced endothelial cells and Rab7 haploinsufficient mice, have provided valuable insights into its role in diseases like pulmonary hypertension, oocyte-related disorders, and diabetic cardiomyopathy, highlighting its potential as a therapeutic target.
References:
1. Wong, Yvette C, Ysselstein, Daniel, Krainc, Dimitri. 2018. Mitochondria-lysosome contacts regulate mitochondrial fission via RAB7 GTP hydrolysis. In Nature, 554, 382-386. doi:10.1038/nature25486. https://pubmed.ncbi.nlm.nih.gov/29364868/
2. Piper, Bryce, Bogamuwa, Srimathi, Hossain, Tanvir, Eckmann, David M, Farkas, Laszlo. 2024. RAB7 deficiency impairs pulmonary artery endothelial function and promotes pulmonary hypertension. In The Journal of clinical investigation, 134, . doi:10.1172/JCI169441. https://pubmed.ncbi.nlm.nih.gov/38015641/
3. Jin, Xin, Wang, Kehan, Wang, Lu, Wu, Tinghe, Li, Jing. 2021. RAB7 activity is required for the regulation of mitophagy in oocyte meiosis and oocyte quality control during ovarian aging. In Autophagy, 18, 643-660. doi:10.1080/15548627.2021.1946739. https://pubmed.ncbi.nlm.nih.gov/34229552/
4. Rodriguez-Furlan, Cecilia, Borna, Rita, Betz, Oliver. 2023. RAB7 GTPases as coordinators of plant endomembrane traffic. In Frontiers in plant science, 14, 1240973. doi:10.3389/fpls.2023.1240973. https://pubmed.ncbi.nlm.nih.gov/37662169/
5. Ke, Jiahan, Pan, Jianan, Lin, Hao, Chang, Alex Chia Yu, Gu, Jun. 2024. Targeting Rab7-Rilp Mediated Microlipophagy Alleviates Lipid Toxicity in Diabetic Cardiomyopathy. In Advanced science (Weinheim, Baden-Wurttemberg, Germany), 11, e2401676. doi:10.1002/advs.202401676. https://pubmed.ncbi.nlm.nih.gov/38837607/
6. Modica, Graziana, Lefrancois, Stephane. 2018. Post-translational modifications: How to modulate Rab7 functions. In Small GTPases, 11, 167-173. doi:10.1080/21541248.2017.1387686. https://pubmed.ncbi.nlm.nih.gov/29099291/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen