C57BL/6JCya-Npdc1em1/Cya
Common Name:
Npdc1-KO
Product ID:
S-KO-20332
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
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Basic Information
Strain Name
Npdc1-KO
Strain ID
KOCMP-18146-Npdc1-B6J-VC
Gene Name
Product ID
S-KO-20332
Gene Alias
NPDC-1
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
2
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Npdc1em1/Cya mice (Catalog S-KO-20332) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000071442
NCBI RefSeq
NM_008721
Target Region
Exon 3~9
Size of Effective Region
~2.7 kb
Detailed Document
Overview of Gene Research
Npdc1, short for neural proliferation differentiation and control-1, is a gene involved in crucial biological processes. It has been associated with neural cell proliferation and differentiation [4]. Its expression pattern and function suggest it may play a role in the development of certain secretion glands as well [4]. In the context of disease, it has emerged as potentially important in conditions like colon cancer and neurodegenerative diseases.
In colon cancer, high Npdc1 expression was correlated with patient prognosis and may be associated with neural infiltration. It could promote tumorigenesis, progression, and chemoresistance through various pathways, indicating its potential as a predictive marker for perineural invasion status, disease-free survival, and overall survival [1]. In the study of retinal progenitor cells, miR-762 was shown to regulate their proliferation and differentiation by targeting Npdc1. Inhibition of Npdc1 by miR-762 positively regulated RPC proliferation and suppressed neuronal differentiation [2]. In the cerebral cortex, Arhgef2 deficiency affected neural differentiation by regulating m6A methylation of Npdc1, among other genes. Loss of Arhgef2 decreased m6A methylation of Npdc1 mRNA, inhibiting its translation, and overexpression of Npdc1 rescued the abnormal phenotypes in knockout mice [3].
In conclusion, Npdc1 is essential for neural cell proliferation and differentiation. Its study through gene-knockout models, as seen in the Arhgef2-related neural differentiation study, has provided insights into its role in neural development and disease. In colon cancer, its potential as a prognostic and predictive marker emphasizes its significance in understanding cancer progression and treatment response.
References:
1. Li, Jiannan, Sun, Yao, Cao, Lanqing, Wang, Fang. 2023. Correlation of NPDC1 Expression and Perineural Invasion Status with Clinicopathological Features in Patients with Colon Cancer. In International journal of general medicine, 16, 4549-4563. doi:10.2147/IJGM.S428590. https://pubmed.ncbi.nlm.nih.gov/37822345/
2. Gao, Huiqin, Ni, Ni, Zhang, Dandan, Liu, Yan, Gu, Ping. 2020. miR-762 regulates the proliferation and differentiation of retinal progenitor cells by targeting NPDC1. In Cell cycle (Georgetown, Tex.), 19, 1754-1767. doi:10.1080/15384101.2020.1777805. https://pubmed.ncbi.nlm.nih.gov/32544377/
3. Zhou, Pei, Qi, Yifei, Fang, Xiang, Kaindl, Angela M, Hu, Hao. 2021. Arhgef2 regulates neural differentiation in the cerebral cortex through mRNA m6A-methylation of Npdc1 and Cend1. In iScience, 24, 102645. doi:10.1016/j.isci.2021.102645. https://pubmed.ncbi.nlm.nih.gov/34142067/
4. Qu, X, Zhang, C, Zhai, Y, Wu, S, He, F. . Characterization and tissue expression of a novel human gene npdc1. In Gene, 264, 37-44. doi:. https://pubmed.ncbi.nlm.nih.gov/11245976/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen