C57BL/6JCya-Pfn2em1/Cya
Common Name
Pfn2-KO
Product ID
S-KO-20340
Backgroud
C57BL/6JCya
Strain ID
KOCMP-18645-Pfn2-B6J-VB
When using this mouse strain in a publication, please cite “Pfn2-KO Mouse (Catalog S-KO-20340) were purchased from Cyagen.”
Product Type
Age
Genotype
Sex
Quantity
Basic Information
Strain Name
Pfn2-KO
Strain ID
KOCMP-18645-Pfn2-B6J-VB
Gene Name
Product ID
S-KO-20340
Gene Alias
Pfn
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
Chr 3
Phenotype
Datasheet
Application
--
Strain Description
Ensembl Number
ENSMUST00000066882
NCBI RefSeq
NM_019410
Target Region
Exon 2
Size of Effective Region
~1.4 kb
Overview of Gene Research
Pfn2, or Profilin 2, is an actin-binding protein. Profilins generally bind to globular (G -)actin and regulate actin filament formation, which is crucial for cell shape, division, and intracellular force generation. Pfn2 is also involved in endocytosis as an actin/dynamin-binding protein and is associated with pathways like the MAPK/JNK pathway [1,2].
In gastric cancer cells, a SIX2/Pfn2 feedback loop was found to enhance cell stemness. SIX2 directly binds to Pfn2's promoter and promotes its activity, while Pfn2 promotes the mRNA stability of SIX2 by recruiting YBX-1, activating the downstream MAPK/JNK pathway [1]. In triple-negative breast cancer, Pfn2 promotes cell proliferation, migration, invasion, and epithelial-to-mesenchymal transition (EMT) [3]. In esophageal squamous cell carcinoma, high Pfn2 expression is associated with poor prognosis, and its down-regulation inhibits cell invasion and migration, and induces an EMT phenotype [4]. In breast cancer, circular RNAs like circ_0008500 and circABCB10 can regulate Pfn2 expression, affecting cell growth, apoptosis, radiosensitivity, and glycolytic metabolism [5,6].
In conclusion, Pfn2 is a key regulator in multiple biological processes and disease conditions. Through functional studies, especially in cancer-related research, Pfn2 has been shown to be involved in cell stemness, proliferation, migration, invasion, and EMT. Understanding the role of Pfn2 in these processes provides potential therapeutic targets for various cancers.
References：
1. Guo, Qianqian, Zhou, Yi, Ni, Haiwei, Zheng, Lufeng, Zhang, Wenzhou. 2024. The SIX2/PFN2 feedback loop promotes the stemness of gastric cancer cells. In Journal of translational medicine, 22, 832. doi:10.1186/s12967-024-05618-5. https://pubmed.ncbi.nlm.nih.gov/39256760/
2. Sangokoya, Carolyn, Blelloch, Robert. 2020. MicroRNA-dependent inhibition of PFN2 orchestrates ERK activation and pluripotent state transitions by regulating endocytosis. In Proceedings of the National Academy of Sciences of the United States of America, 117, 20625-20635. doi:10.1073/pnas.2002750117. https://pubmed.ncbi.nlm.nih.gov/32788350/
3. Ling, Yuwei, Cao, Qi, Liu, Yihan, Kang, Hua, Chen, Zhenwen. 2020. Profilin 2 (PFN2) promotes the proliferation, migration, invasion and epithelial-to-mesenchymal transition of triple negative breast cancer cells. In Breast cancer (Tokyo, Japan), 28, 368-378. doi:10.1007/s12282-020-01169-x. https://pubmed.ncbi.nlm.nih.gov/33047272/
4. Cui, Xiao-Bin, Zhang, Shu-Mao, Xu, Yue-Xun, Chen, Yun-Zhao, Li, Feng. 2016. PFN2, a novel marker of unfavorable prognosis, is a potential therapeutic target involved in esophageal squamous cell carcinoma. In Journal of translational medicine, 14, 137. doi:10.1186/s12967-016-0884-y. https://pubmed.ncbi.nlm.nih.gov/27188458/
5. Kong, Deyou, Shen, Dongxing, Liu, Zhikun, Zhang, Jian, Geng, Cuizhi. 2022. Circ_0008500 Knockdown Improves Radiosensitivity and Inhibits Tumorigenesis in Breast Cancer Through the miR-758-3p/PFN2 Axis. In Journal of mammary gland biology and neoplasia, 27, 37-52. doi:10.1007/s10911-022-09514-w. https://pubmed.ncbi.nlm.nih.gov/35239064/
6. Zhao, Yue, Zhong, Rui, Deng, Chaoyue, Zhou, Zhenlin. 2020. Circle RNA circABCB10 Modulates PFN2 to Promote Breast Cancer Progression, as Well as Aggravate Radioresistance Through Facilitating Glycolytic Metabolism Via miR-223-3p. In Cancer biotherapy & radiopharmaceuticals, 36, 477-490. doi:10.1089/cbr.2019.3389. https://pubmed.ncbi.nlm.nih.gov/32522014/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen
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