C57BL/6JCya-Tulp1em1/Cya
Common Name:
Tulp1-KO
Product ID:
S-KO-20342
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
Tulp1-KO
Strain ID
KOCMP-22157-Tulp1-B6J-VB
Gene Name
Product ID
S-KO-20342
Gene Alias
Tulp1l
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
17
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Tulp1em1/Cya mice (Catalog S-KO-20342) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000041819
NCBI RefSeq
NM_021478
Target Region
Exon 6~8
Size of Effective Region
~1.6 kb
Detailed Document
Overview of Gene Research
Tulp1, short for tubby-like protein 1, is a photoreceptor-specific protein crucial for protein trafficking in the retina. It localizes to the inner segment (IS) and synapse of photoreceptor cells. Tulp1 likely functions as an adapter molecule, linking vesicles to molecular motors and the cytoskeletal scaffold, which is essential for the proper localization of various photoreceptor proteins [2].
In zebrafish models, knockout of tulp1a led to mislocalization of UV cone opsins and specific UV cone degeneration, while tulp1b knockout caused mislocalization of rod opsins and rod-cone degeneration. The double knockout (tulp1-dKO) zebrafish showed opsin mislocalization in all photoreceptor types, severe early-onset degeneration, reduced photoreceptor cilium length, down-regulation of tektin2 (a ciliary and flagellar microtubule component), and activation of ferroptosis [1]. In Tulp1-deficient mice, the localization of photoreceptor disc component (PRCD) to photoreceptor outer segment discs was altered [3].
In conclusion, Tulp1 is vital for opsin trafficking and cilia structure in photoreceptor cells. Gene knockout models in zebrafish and mice have revealed its role in preventing early-onset retinal degeneration, highlighting its significance in understanding the pathogenesis of retinal dystrophies [1,3].
References:
1. Jia, Danna, Gao, Pan, Lv, Yuexia, Liu, Mugen, Ren, Xiang. 2022. Tulp1 deficiency causes early-onset retinal degeneration through affecting ciliogenesis and activating ferroptosis in zebrafish. In Cell death & disease, 13, 962. doi:10.1038/s41419-022-05372-w. https://pubmed.ncbi.nlm.nih.gov/36396940/
2. Ebke, Lindsey A, Sinha, Satyabrata, Pauer, Gayle J T, Hagstrom, Stephanie A. 2021. Photoreceptor Compartment-Specific TULP1 Interactomes. In International journal of molecular sciences, 22, . doi:10.3390/ijms22158066. https://pubmed.ncbi.nlm.nih.gov/34360830/
3. Remez, Lital, Cohen, Ben, Nevet, Mariela J, Rizel, Leah, Ben-Yosef, Tamar. 2020. TULP1 and TUB Are Required for Specific Localization of PRCD to Photoreceptor Outer Segments. In International journal of molecular sciences, 21, . doi:10.3390/ijms21228677. https://pubmed.ncbi.nlm.nih.gov/33213002/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen