C57BL/6JCya-St3gal6em1/Cya
Common Name:
St3gal6-KO
Product ID:
S-KO-20349
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
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Basic Information
Strain Name
St3gal6-KO
Strain ID
KOCMP-54613-St3gal6-B6J-VB
Gene Name
Product ID
S-KO-20349
Gene Alias
1700023B24Rik; Siat10; St3galVI
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
16
Phenotype
Document
Application
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Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-St3gal6em1/Cya mice (Catalog S-KO-20349) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000137035
NCBI RefSeq
NM_018784
Target Region
Exon 5
Size of Effective Region
~1.2 kb
Detailed Document
Overview of Gene Research
St3gal6, short for ST3 β -galactoside α2,3 -sialyltransferase 6, is a significant glycosyltransferase. It is responsible for adding α2,3 -linked sialic acid to substrates, generating glycan structures, and thus plays a role in sialylation, an essential form of glycosylation [2]. Sialylation is associated with multiple biological processes, and St3gal6 may be involved in pathways like PI3K/Akt, which are related to cancer progression and cell autophagy [1,2].
In multiple myeloma, knockdown of St3gal6 led to a significant reduction in levels of α -2,3 -linked sialic acid on the cell surface, along with decreased adhesion to bone marrow stromal cells and fibronectin, and reduced transendothelial migration in vitro. In vivo, it showed reduced homing and engraftment of St3gal6 -knockdown cells to the bone marrow niche, decreased tumor burden, and prolonged survival [5].
In hepatocellular carcinoma, upregulation of St3gal6 was found to correlate with cell proliferation, migration, and invasion ability, and miR -26a negatively regulated St3gal6, suppressing these cellular activities through the Akt/mTOR pathway [3].
In lung adenocarcinoma, deficiency of St3gal6 promoted cell invasiveness with activated EGFR/MAPK signaling [4].
In hepatic encephalopathy, silencing St3gal6 and blocking or degrading α2,3 -sialylated glycans inhibited autophagy, and St3gal6 regulated autophagy through the HSPB8-BAG3 complex [2].
In conclusion, St3gal6 plays crucial roles in multiple biological processes and disease conditions. Through gene-knockout or knockdown models, its functions in cancer cell behavior such as proliferation, migration, invasion, as well as in cell adhesion, autophagy, and related signaling pathways have been revealed. These findings contribute to understanding the mechanisms of diseases like multiple myeloma, hepatocellular carcinoma, lung adenocarcinoma, and hepatic encephalopathy, providing potential targets for treatment.
References:
1. Hu, Jialei, Shan, Yujia, Ma, Jia, Jiang, Liqun, Jia, Li. 2019. LncRNA ST3Gal6-AS1/ST3Gal6 axis mediates colorectal cancer progression by regulating α-2,3 sialylation via PI3K/Akt signaling. In International journal of cancer, 145, 450-460. doi:10.1002/ijc.32103. https://pubmed.ncbi.nlm.nih.gov/30613961/
2. Li, Xiaocheng, Xiao, Yaqing, Li, Pengfei, Bian, Huijie, Li, Zheng. 2024. Sialyltransferase ST3GAL6 silencing reduces α2,3-sialylated glycans to regulate autophagy by decreasing HSPB8-BAG3 in the brain with hepatic encephalopathy. In Journal of Zhejiang University. Science. B, 25, 485-498. doi:10.1631/jzus.B2300917. https://pubmed.ncbi.nlm.nih.gov/38910494/
3. Sun, Mingming, Zhao, Xuzi, Liang, Leilei, Lv, Hao, Zhao, Yongfu. . Sialyltransferase ST3GAL6 mediates the effect of microRNA-26a on cell growth, migration, and invasion in hepatocellular carcinoma through the protein kinase B/mammalian target of rapamycin pathway. In Cancer science, 108, 267-276. doi:10.1111/cas.13128. https://pubmed.ncbi.nlm.nih.gov/27906498/
4. Li, Jiaxuan, Long, Yiming, Sun, Jingya, Huang, Ruimin, Yan, Jun. 2022. Comprehensive landscape of the ST3GAL family reveals the significance of ST3GAL6-AS1/ST3GAL6 axis on EGFR signaling in lung adenocarcinoma cell invasion. In Frontiers in cell and developmental biology, 10, 931132. doi:10.3389/fcell.2022.931132. https://pubmed.ncbi.nlm.nih.gov/36092699/
5. Glavey, Siobhan V, Manier, Salomon, Natoni, Alessandro, Ghobrial, Irene M, O'Dwyer, Michael E. 2014. The sialyltransferase ST3GAL6 influences homing and survival in multiple myeloma. In Blood, 124, 1765-76. doi:10.1182/blood-2014-03-560862. https://pubmed.ncbi.nlm.nih.gov/25061176/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen