C57BL/6JCya-Ciitaem1/Cya
Common Name:
Ciita-KO
Product ID:
S-KO-20350
Background:
C57BL/6JCya
Product Type
Age
Genotype
Sex
Quantity
Price:
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Basic Information
Strain Name
Ciita-KO
Strain ID
KOCMP-12265-Ciita-B6J-VB
Gene Name
Product ID
S-KO-20350
Gene Alias
C2ta; EG669998; Gm9475; Mhc2ta
Background
C57BL/6JCya
NCBI ID
Modification
Conventional knockout
Chromosome
16
Phenotype
Document
Application
--
Note: When using this mouse strain in a publication, please cite “C57BL/6JCya-Ciitaem1/Cya mice (Catalog S-KO-20350) were purchased from Cyagen.”
Strain Description
Ensembl Number
ENSMUST00000230395
NCBI RefSeq
NM_001302618
Target Region
Exon 2~3
Size of Effective Region
~1.1 kb
Detailed Document
Overview of Gene Research
Ciita, short for MHC class II transactivator, is a master regulator of MHC class II (MHC-II) gene transcription. As a member of the NLR (nucleotide-binding domain, leucine-rich-repeat containing) protein family, it plays a critical role in the activation and regulation of adaptive immunity through antigen presentation to CD4+ T cells [1,2,3]. The strict regulation of MHC-II expression by Ciita is crucial for proper immune responses. Genetic models, such as KO/CKO mouse models, are valuable for studying Ciita's functions.
FBXO11, an E3 ligase, regulates Ciita protein level through ubiquitination-mediated degradation, thereby affecting MHC-II expression at promoter, transcriptional, and surface levels [1]. Human and mouse FBXO11-deficient cells display increased levels of MHC-II and related genes, indicating the negative regulatory role of FBXO11 on Ciita-mediated MHC-II expression. In cancer, the expression of FBXO11, along with Ciita, is associated with prognosis [1].
In a rat model of Parkinson's disease, reduced Ciita levels significantly increase α-Syn pathology, nigrostriatal neurodegeneration and behavioral deficits, suggesting that Ciita regulates susceptibility to PD-like pathology through changes in resident and peripheral immune cells and TNF levels [4]. Also, in a Chinese family, a novel missense variant in the CIITA gene was identified in sisters with ovarian endometriosis, and the mutation affected cell migration and invasion ability, demonstrating that CIITA variants may contribute to the development of endometriosis [5].
In conclusion, Ciita is essential for regulating MHC-II gene expression and thus adaptive immunity. Studies using gene knockout models have revealed its significance in diseases like cancer, Parkinson's disease, and endometriosis. These findings contribute to understanding the underlying mechanisms of these diseases and may offer potential therapeutic targets.
References:
1. Kasuga, Yusuke, Ouda, Ryota, Watanabe, Masashi, Hatakeyama, Shigetsugu, Kobayashi, Koichi S. 2023. FBXO11 constitutes a major negative regulator of MHC class II through ubiquitin-dependent proteasomal degradation of CIITA. In Proceedings of the National Academy of Sciences of the United States of America, 120, e2218955120. doi:10.1073/pnas.2218955120. https://pubmed.ncbi.nlm.nih.gov/37279268/
2. Forlani, Greta, Shallak, Mariam, Gatta, Andrea, Shaik, Amruth K B, Accolla, Roberto S. 2023. The NLR member CIITA: Master controller of adaptive and intrinsic immunity and unexpected tool in cancer immunotherapy. In Biomedical journal, 46, 100631. doi:10.1016/j.bj.2023.100631. https://pubmed.ncbi.nlm.nih.gov/37467968/
3. León Machado, Jorge Alfonso, Steimle, Viktor. 2021. The MHC Class II Transactivator CIITA: Not (Quite) the Odd-One-Out Anymore among NLR Proteins. In International journal of molecular sciences, 22, . doi:10.3390/ijms22031074. https://pubmed.ncbi.nlm.nih.gov/33499042/
4. Fredlund, Filip, Jimenez-Ferrer, Itzia, Grabert, Kathleen, Luk, Kelvin, Swanberg, Maria. . Ciita Regulates Local and Systemic Immune Responses in a Combined rAAV-α-synuclein and Preformed Fibril-Induced Rat Model for Parkinson's Disease. In Journal of Parkinson's disease, 14, 693-711. doi:10.3233/JPD-240062. https://pubmed.ncbi.nlm.nih.gov/38728204/
5. Zhu, Ying, Pan, Hong, Han, Yang, Liu, Kaijiang, Wang, Binbin. 2022. Novel missense variant of CIITA contributing to endometriosis. In Reproductive biomedicine online, 45, 544-551. doi:10.1016/j.rbmo.2022.05.011. https://pubmed.ncbi.nlm.nih.gov/35835655/
Quality Control Standard
Sperm Test
Pre-cryopreservation: Measurement of sperm concentration, determination of sperm viability.
Post-cryopreservation: A vial of cryopreserved sperms is selected for in-vitro fertilization from each batch.
Environmental Standards:SPF
Available Region:Global
Source:Cyagen